論文 - 詳細
| RRC ID | 15393 |
|---|---|
| 著者 | Shin JE, DiAntonio A. |
| タイトル | Highwire regulates guidance of sister axons in the Drosophila mushroom body. |
| ジャーナル | J Neurosci |
| Abstract |
Axons often form synaptic contacts with multiple targets by extending branches along different paths. PHR (Pam/Highwire/RPM-1) family ubiquitin ligases are important regulators of axon development, with roles in axon outgrowth, target selection, and synapse formation. Here we report the function of Highwire, the Drosophila member of the PHR family, in promoting the segregation of sister axons during mushroom body (MB) formation. Loss of highwire results in abnormal development of the axonal lobes in the MB, leading to thinned and shortened lobes. The highwire defect is attributable to guidance errors after axon branching, in which sister axons that should target different lobes instead extend together into the same lobe. The highwire mutant MB displays elevation in the level of the MAPKKK Wallenda/DLK (dual leucine zipper kinase), a previously identified substrate of Highwire, and genetic suppression studies show that Wallenda/DLK is required for the highwire MB phenotype. The highwire lobe defect is limited to α/β lobe axons, but transgenic expression of highwire in the pioneering α'/β' neurons rescues the phenotype. Mosaic analysis further shows that α/β axons of highwire mutant clones develop normally, demonstrating a non-cell-autonomous role of Highwire for axon guidance. Genetic interaction studies suggest that Highwire and Plexin A signals may interact to regulate normal morphogenesis of α/β axons. |
| 巻・号 | 31(48) |
| ページ | 17689-700 |
| 公開日 | 2011-11-30 |
| DOI | 10.1523/JNEUROSCI.3902-11.2011 |
| PII | 31/48/17689 |
| PMID | 22131429 |
| PMC | PMC3457808 |
| MeSH | Animals Animals, Genetically Modified Axons / physiology* Cell Adhesion Molecules / genetics Cell Adhesion Molecules / metabolism Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster Mushroom Bodies / growth & development Mushroom Bodies / metabolism* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Neurons / physiology Phenotype |
| IF | 5.674 |
| 引用数 | 25 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | NP2748-Gal4 (DGRC#104309) and NP7175-Gal4 (DGRC#114120) |