論文 - 詳細
| RRC ID | 15551 |
|---|---|
| 著者 | Resnik-Docampo M, de Celis JF. |
| タイトル | MAP4K3 is a component of the TORC1 signalling complex that modulates cell growth and viability in Drosophila melanogaster. |
| ジャーナル | PLoS One |
| Abstract |
BACKGROUND:MAP4K3 is a conserved Ser/Thr kinase that has being found in connection with several signalling pathways, including the Imd, EGFR, TORC1 and JNK modules, in different organisms and experimental assays. We have analyzed the consequences of changing the levels of MAP4K3 expression in the development of the Drosophila wing, a convenient model system to characterize gene function during epithelial development. METHODOLOGY AND PRINCIPAL FINDINGS:Using loss-of-function mutants and over-expression conditions we find that MAP4K3 activity affects cell growth and viability in the Drosophila wing. These requirements are related to the modulation of the TORC1 and JNK signalling pathways, and are best detected when the larvae grow in a medium with low protein concentration (TORC1) or are exposed to irradiation (JNK). We also show that MAP4K3 display strong genetic interactions with different components of the InR/Tor signalling pathway, and can interact directly with the GTPases RagA and RagC and with the multi-domain kinase Tor. CONCLUSIONS AND SIGNIFICANCE:We suggest that MAP4K3 has two independent functions during wing development, one related to the activation of the JNK pathway in response to stress and other in the assembling or activation of the TORC1 complex, being critical to modulate cellular responses to changes in nutrient availability. |
| 巻・号 | 6(1) |
| ページ | e14528 |
| 公開日 | 2011-1-18 |
| DOI | 10.1371/journal.pone.0014528 |
| PMID | 21267071 |
| PMC | PMC3022576 |
| MeSH | Animals Cell Proliferation* Cell Survival Drosophila Proteins / metabolism Drosophila Proteins / pharmacology Drosophila Proteins / physiology* Drosophila melanogaster / cytology Drosophila melanogaster / physiology* Female Gene Expression Regulation, Developmental* Intracellular Signaling Peptides and Proteins / metabolism Intracellular Signaling Peptides and Proteins / pharmacology JNK Mitogen-Activated Protein Kinases / metabolism Protein Kinases / metabolism Protein Kinases / pharmacology Protein Serine-Threonine Kinases / metabolism Protein Serine-Threonine Kinases / physiology* Signal Transduction* TOR Serine-Threonine Kinases Transcription Factors / metabolism Wings, Animal / cytology |
| IF | 2.74 |
| 引用数 | 16 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | 5092R-2 4320R-2 10539R-3 |