Reference - Detail
| RRC ID | 15579 |
|---|---|
| Author | Johnson RI, Sedgwick A, D'Souza-Schorey C, Cagan RL. |
| Title | Role for a Cindr-Arf6 axis in patterning emerging epithelia. |
| Journal | Mol Biol Cell |
| Abstract |
Patterning of the Drosophila pupal eye is characterized by precise cell movements. In this paper, we demonstrate that these movements require an Arf regulatory cycle that connects surface receptors to actin-based movement. dArf6 activity-regulated by the Arf GTPase-activating proteins (ArfGAPs) dAsap and dArfGAP3 and the Arf GTP exchange factors Schizo and dPsd-promoted large cellular extensions; time-lapse microscopy indicated that these extensions presage cell rearrangements into correct epithelial niches. During this process, the Drosophila eye also requires interactions between surface Neph1/nephrin adhesion receptors Roughest and Hibris, which bind the adaptor protein Cindr (CD2AP). We provide evidence that Cindr forms a physical complex with dArfGAP3 and dAsap. Our data suggest this interaction sequesters ArfGAP function to liberate active dArf6 elsewhere in the cell. We propose that a Neph1/nephrin-Cindr/ArfGAP complex accumulates to limit local Arf6 activity and stabilize adherens junctions. Our model therefore links surface adhesion via an Arf6 regulatory cascade to dynamic modeling of the cytoskeleton, accounting for precise cell movements that organize the functional retinal field. Further, we demonstrate a similar relationship between the mammalian Cindr orthologue CD2AP and Arf6 activity in cell motility assays. We propose that this Cindr/CD2AP-mediated regulation of Arf6 is a widely used mechanism in emerging epithelia. |
| Volume | 22(23) |
| Pages | 4513-26 |
| Published | 2011-12-1 |
| DOI | 10.1091/mbc.E11-04-0305 |
| PII | mbc.E11-04-0305 |
| PMID | 21976699 |
| PMC | PMC3226471 |
| MeSH | ADP-Ribosylation Factors / genetics ADP-Ribosylation Factors / metabolism Actins / metabolism Adherens Junctions / metabolism Animals Animals, Genetically Modified Body Patterning / physiology* Cell Adhesion Molecules, Neuronal / genetics Cell Adhesion Molecules, Neuronal / metabolism Cell Movement Drosophila / growth & development* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Epithelium / growth & development* Eye / cytology* Eye / growth & development Eye Proteins / genetics Eye Proteins / metabolism Guanine Nucleotide Exchange Factors / genetics Guanine Nucleotide Exchange Factors / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Microfilament Proteins / metabolism* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Pupa |
| IF | 3.791 |
| Times Cited | 16 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Blogs |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 31158R-2 |