論文 - 詳細
| RRC ID | 15638 |
|---|---|
| 著者 | Ihara K, Narusawa A, Maruyama K, Takeguchi M, Kouyama T. |
| タイトル | A halorhodopsin-overproducing mutant isolated from an extremely haloalkaliphilic archaeon Natronomonas pharaonis. |
| ジャーナル | FEBS Lett |
| Abstract |
The halorhodopsin (hR)-overproducing mutant strain KM-1 was isolated from the extremely haloalkaliphilic archaeon Natronomonas pharaonis type strain DSM2160(T). hR-enriched membranes were easily obtained by washing the cells with distilled water. The membranes were claret colored owing to two pigments: hR and bacterioruberin. The hR component in the absorption spectra changed from blue to purple upon the addition of Cl(-) and had a K(m) value of 1.7 mM. Overexpression of hR in strain KM-1 might be caused by the point mutation Asp324-->Asn in the bacteriorhodopsin activator homologues of N. pharaonis. The mutation changed the hR-expression pattern from inducible to constitutive in the late exponential phase. |
| 巻・号 | 582(19) |
| ページ | 2931-6 |
| 公開日 | 2008-8-20 |
| DOI | 10.1016/j.febslet.2008.07.030 |
| PII | S0014-5793(08)00621-2 |
| PMID | 18671971 |
| MeSH | Amino Acid Substitution Asparagine / genetics Asparagine / metabolism Aspartic Acid / genetics Aspartic Acid / metabolism Cell Membrane / chemistry Cell Membrane / metabolism Gene Expression Regulation, Archaeal Halobacteriaceae / genetics Halobacteriaceae / isolation & purification Halobacteriaceae / metabolism* Halorhodopsins / biosynthesis* Halorhodopsins / chemistry Halorhodopsins / genetics Mutation |
| IF | 3.057 |
| 引用数 | 22 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 8858 |