論文 - 詳細
| RRC ID | 15665 |
|---|---|
| 著者 | Yanagida F, Srionnual S, Chen YS. |
| タイトル | Isolation and characteristics of lactic acid bacteria from koshu vineyards in Japan. |
| ジャーナル | Lett Appl Microbiol |
| Abstract |
AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in the vineyards where koshu grapes, a primary wine grape cultivar in Japan, are grown. METHODS AND RESULTS:Sixty samples, including leaves, undamaged grape berries and soil under damaged berries, were collected at four koshu vineyards in Yamanashi Prefecture, Japan. One hundred and 15 acid-producing cultures were isolated from these samples, and the isolates were divided into classes by phenotype and then into groups by restriction fragment length polymorphism analysis and sequencing of 16S ribosomal DNA (rDNA). Phenotypic and biochemical characteristics identified seven different bacterial groups (A to G). Lactococcus lactis ssp. lactis was the most abundant type of LAB distributed in three koshu vineyards, and Leuconostoc pseudomesenteroides was the most abundant LAB found in the remaining vineyard. Forty-six isolates produced bacteriocin-like inhibitory substances (BLIS) against the indicator strain Lactobacillus sakei JCM 1157(T). CONCLUSIONS:These results suggest that various LAB are distributed in koshu vineyards, of which a large number produce BLIS. SIGNIFICANCE AND IMPACT OF THE STUDY:This is the first report describing the distribution and varieties of LAB that exist in koshu vineyards. |
| 巻・号 | 47(2) |
| ページ | 134-9 |
| 公開日 | 2008-8-1 |
| DOI | 10.1111/j.1472-765X.2008.02398.x |
| PII | LAM2398 |
| PMID | 18673430 |
| MeSH | Antibiosis Bacteriocins / biosynthesis DNA Fingerprinting DNA, Bacterial / chemistry DNA, Bacterial / genetics DNA, Ribosomal / chemistry DNA, Ribosomal / genetics Fruit / microbiology Genes, rRNA Genotype Japan Lactic Acid / metabolism* Lactobacillus / classification* Lactobacillus / isolation & purification* Lactobacillus / metabolism Lactococcus lactis / classification* Lactococcus lactis / isolation & purification* Lactococcus lactis / metabolism Leuconostoc / classification* Leuconostoc / isolation & purification* Leuconostoc / metabolism Molecular Sequence Data Phylogeny Plant Leaves / microbiology Polymorphism, Restriction Fragment Length RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Soil Microbiology Vitis / microbiology* Wine |
| IF | 2.173 |
| 引用数 | 27 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 一般微生物 | JCM 1157 |