| RRC ID |
1584
|
| Author |
Matsuura K, Wada H, Nagai T, Iijima Y, Minamino T, Sano M, Akazawa H, Molkentin JD, Kasanuki H, Komuro I.
|
| Title |
Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle.
|
| Journal |
J Cell Biol
|
| Abstract |
The concept of the plasticity or transdifferentiation of adult stem cells has been challenged by the phenomenon of cell fusion. In this work, we examined whether neonatal cardiomyocytes fuse with various somatic cells including endothelial cells, cardiac fibroblasts, bone marrow cells, and endothelial progenitor cells spontaneously in vitro. When cardiomyocytes were cocultured with endothelial cells or cardiac fibroblasts, they fused and showed phenotypes of cardiomyocytes. Furthermore, cardiomyocytes reentered the G2-M phase in the cell cycle after fusing with proliferative noncardiomyocytes. Transplanted endothelial cells or skeletal muscle-derived cells fused with adult cardiomyocytes in vivo. In the cryoinjured heart, there were Ki67-positive cells that expressed both cardiac and endothelial lineage marker proteins. These results suggest that cardiomyocytes fuse with other cells and enter the cell cycle by maintaining their phenotypes.
|
| Volume |
167(2)
|
| Pages |
351-63
|
| Published |
2004-10-25
|
| DOI |
10.1083/jcb.200312111
|
| PII |
jcb.200312111
|
| PMID |
15492039
|
| PMC |
PMC2172538
|
| MeSH |
Adenoviridae / genetics
Animals
Animals, Genetically Modified
Cell Communication
Cell Cycle
Cell Differentiation
Cell Division
Cell Lineage
Cell Proliferation
Cell Transplantation
Cells, Cultured
Endothelium, Vascular / cytology
Endothelium, Vascular / metabolism
Fibroblasts / metabolism
G2 Phase
Green Fluorescent Proteins / metabolism
Immunohistochemistry
Ki-67 Antigen / biosynthesis
Lac Operon
Male
Mice
Models, Genetic
Muscle, Skeletal / cytology
Myocytes, Cardiac / metabolism*
Nocodazole / pharmacology
Phenotype
Rats
Rats, Wistar
Recombination, Genetic
Time Factors
Transgenes
|
| IF |
8.811
|
| Times Cited |
93
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| DNA material |
AxCANCre (RDB01748) |
