RRC ID 17449
著者 Ugwu CU, Tokiwa Y, Ichiba T.
タイトル Production of (R)-3-hydroxybutyric acid by fermentation and bioconversion processes with Azohydromonas lata.
ジャーナル Bioresour Technol
Abstract Feasibility of producing (R)-3-hydroxybutyric acid ((R)-3-HB) using wild type Azohydromonas lata and its mutants (derived by UV mutation) was investigated. A. lata mutant (M5) produced 780 m g/l in the culture broth when sucrose was used as the carbon source. M5 was further studied in terms of its specificity with various bioconversion substrates for production of (R)-3-HB. (R)-3-HB concentration produced in the culture broth by M5 mutant was 2.7-fold higher than that of the wild type strain when sucrose (3% w/v) and (R,S)-1,3-butanediol (3% v/v) were used as carbon source and bioconversion substrate, respectively. Bioconversion of resting cells (M5) with glucose (1% v/w), ethylacetoacetate (2% v/v), and (R,S)-1,3-butanediol (3% v/v), resulted in (R)-3-HB concentrations of 6.5 g/l, 7.3g/l and 8.7 g/l, respectively.
巻・号 102(12)
ページ 6766-8
公開日 2011-6-1
DOI 10.1016/j.biortech.2011.03.073
PII S0960-8524(11)00430-5
PMID 21507620
MeSH 3-Hydroxybutyric Acid / biosynthesis* Alcaligenaceae / genetics Alcaligenaceae / metabolism* Biotechnology / methods* Butylene Glycols Culture Media Fermentation Mutagenesis / radiation effects Mutation / genetics Species Specificity Sucrose Ultraviolet Rays
IF 7.539
引用数 11
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AGRICULTURAL ENGINEERING ENERGY & FUELS
リソース情報
一般微生物 JCM 20724