Reference - Detail
|Author||Vandamme J, Lettier G, Sidoli S, Di Schiavi E, Nørregaard Jensen O, Salcini AE.|
|Title||The C. elegans H3K27 demethylase UTX-1 is essential for normal development, independent of its enzymatic activity.|
Epigenetic modifications influence gene expression and provide a unique mechanism for fine-tuning cellular differentiation and development in multicellular organisms. Here we report on the biological functions of UTX-1, the Caenorhabditis elegans homologue of mammalian UTX, a histone demethylase specific for H3K27me2/3. We demonstrate that utx-1 is an essential gene that is required for correct embryonic and postembryonic development. Consistent with its homology to UTX, UTX-1 regulates global levels of H3K27me2/3 in C. elegans. Surprisingly, we found that the catalytic activity is not required for the developmental function of this protein. Biochemical analysis identified UTX-1 as a component of a complex that includes SET-16(MLL), and genetic analysis indicates that the defects associated with loss of UTX-1 are likely mediated by compromised SET-16/UTX-1 complex activity. Taken together, these results demonstrate that UTX-1 is required for many aspects of nematode development; but, unexpectedly, this function is independent of its enzymatic activity.
|MeSH||Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans* / growth & development Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Differentiation Embryonic Development / genetics* Epigenesis, Genetic Fertility / genetics Gene Expression Regulation, Developmental Histone Demethylases / genetics Histone Demethylases / metabolism Histones* / genetics Histones* / metabolism Jumonji Domain-Containing Histone Demethylases / genetics Jumonji Domain-Containing Histone Demethylases / metabolism Methylation RNA Interference|
|WOS Category||GENETICS & HEREDITY|
|C.elegans||tm3136 and tm3118|