| RRC ID |
18
|
| Author |
Mikami Y, Okano H, Sakaguchi M, Nakamura M, Shimazaki T, Okano HJ, Kawakami Y, Toyama Y, Toda M.
|
| Title |
Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery.
|
| Journal |
J Neurosci Res
|
| Abstract |
We report a treatment for spinal cord injury involving implantation of dendritic cells (DCs), which act as antigen-presenting cells in the immune system. The novel mechanisms underlying this treatment produce functional recovery. Among the immune cells tested, DCs showed the strongest activity inducing proliferation and survival of neural stem/progenitor cells (NSPCs) in vitro. Furthermore, in DC-implanted adult mice, endogenous NSPCs in the injured spinal cord were activated for mitotic de novo neurogenesis. These DCs produced neurotrophin-3 and activated endogenous microglia in the injured spinal cord. Behavioral analysis revealed the locomotor functions of DC-implanted mice to have recovered significantly as compared to those of control mice. Our results suggest that DC-implantation exerts trophic effects, including activation of endogenous NSPCs, leading to repair of the injured adult spinal cord.
|
| Volume |
76(4)
|
| Pages |
453-65
|
| Published |
2004-5-15
|
| DOI |
10.1002/jnr.20086
|
| PMID |
15114617
|
| MeSH |
Animals
Behavior, Animal
Biotin / analogs & derivatives*
Biotin / metabolism
Bromodeoxyuridine / metabolism
Cell Count / methods
Cell Survival
Dendritic Cells / transplantation*
Dextrans / metabolism
Embryo, Mammalian
Enzyme-Linked Immunosorbent Assay / methods
Female
Glial Fibrillary Acidic Protein / metabolism
Green Fluorescent Proteins
Immunoenzyme Techniques / methods
Immunohistochemistry / methods
In Situ Nick-End Labeling / methods
Intermediate Filament Proteins / genetics
Locomotion / physiology
Luminescent Proteins / genetics
Macrophage-1 Antigen / metabolism
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Transgenic
Microglia / metabolism
Nerve Growth Factors / genetics
Nerve Growth Factors / metabolism
Nerve Regeneration*
Nerve Tissue Proteins / genetics
Nestin
Neurons / physiology*
Neurotrophin 3 / metabolism
Phosphopyruvate Hydratase / metabolism
RNA, Messenger / biosynthesis
Recovery of Function*
Reverse Transcriptase Polymerase Chain Reaction / methods
Spinal Cord Injuries / therapy*
Stem Cells / physiology*
Time Factors
|
| IF |
4.139
|
| Times Cited |
57
|
|
WOS Category
|
NEUROSCIENCES
|
| Resource |
| Mice |
C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb |
