Author Mikami Y, Okano H, Sakaguchi M, Nakamura M, Shimazaki T, Okano HJ, Kawakami Y, Toyama Y, Toda M.
Title Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery.
Journal J Neurosci Res
Abstract We report a treatment for spinal cord injury involving implantation of dendritic cells (DCs), which act as antigen-presenting cells in the immune system. The novel mechanisms underlying this treatment produce functional recovery. Among the immune cells tested, DCs showed the strongest activity inducing proliferation and survival of neural stem/progenitor cells (NSPCs) in vitro. Furthermore, in DC-implanted adult mice, endogenous NSPCs in the injured spinal cord were activated for mitotic de novo neurogenesis. These DCs produced neurotrophin-3 and activated endogenous microglia in the injured spinal cord. Behavioral analysis revealed the locomotor functions of DC-implanted mice to have recovered significantly as compared to those of control mice. Our results suggest that DC-implantation exerts trophic effects, including activation of endogenous NSPCs, leading to repair of the injured adult spinal cord.
Volume 76(4)
Pages 453-65
Published 2004-5-15
DOI 10.1002/jnr.20086
PMID 15114617
MeSH Animals Behavior, Animal Biotin / analogs & derivatives* Biotin / metabolism Bromodeoxyuridine / metabolism Cell Count / methods Cell Survival Dendritic Cells / transplantation* Dextrans / metabolism Embryo, Mammalian Enzyme-Linked Immunosorbent Assay / methods Female Glial Fibrillary Acidic Protein / metabolism Green Fluorescent Proteins Immunoenzyme Techniques / methods Immunohistochemistry / methods In Situ Nick-End Labeling / methods Intermediate Filament Proteins / genetics Locomotion / physiology Luminescent Proteins / genetics Macrophage-1 Antigen / metabolism Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Transgenic Microglia / metabolism Nerve Growth Factors / genetics Nerve Growth Factors / metabolism Nerve Regeneration* Nerve Tissue Proteins / genetics Nestin Neurons / physiology* Neurotrophin 3 / metabolism Phosphopyruvate Hydratase / metabolism RNA, Messenger / biosynthesis Recovery of Function* Reverse Transcriptase Polymerase Chain Reaction / methods Spinal Cord Injuries / therapy* Stem Cells / physiology* Time Factors
IF 4.139
Times Cited 57
Mice C57BL/6-Tg(CAG-EGFP)C14-Y01-FM131Osb