論文 - 詳細
| RRC ID | 18166 |
|---|---|
| 著者 | Kanada S, Nishiyama C, Nakano N, Suzuki R, Maeda K, Hara M, Kitamura N, Ogawa H, Okumura K. |
| タイトル | Critical role of transcription factor PU.1 in the expression of CD80 and CD86 on dendritic cells. |
| ジャーナル | Blood |
| Abstract |
In this study, we investigated the role of a transcription factor, PU.1, in the regulation of CD80 and CD86 expression in dendritic cells (DCs). A chromatin immunoprecipitation assay revealed that PU.1 is constitutively bound to the CD80 and CD86 promoters in bone marrow-derived DCs. In addition, co-expression of PU.1 resulted in the transactivation of the CD80 and CD86 promoters in a reporter assay. The binding of PU.1 to cis-enhancing regions was confirmed by electromobility gel-shift assay. As expected, inhibition of PU.1 expression by short interfering RNA (siRNA) in bone marrow-derived DCs resulted in marked down-regulation of CD80 and CD86 expression. Moreover, overexpression of PU.1 in murine bone marrow-derived lineage-negative cells induced the expression of CD80 and CD86 in the absence of monocyte/DC-related growth factors and/or cytokines. Based on these results, we conclude that PU.1 is a critical factor for the expression of CD80 and CD86. We also found that subcutaneous injection of PU.1 siRNA or topical application of a cream-emulsified PU.1 siRNA efficiently inhibited murine contact hypersensitivity. Our results suggest that PU.1 is a potential target for the treatment of immune-related diseases. |
| 巻・号 | 117(7) |
| ページ | 2211-22 |
| 公開日 | 2011-2-17 |
| DOI | 10.1182/blood-2010-06-291898 |
| PII | S0006-4971(20)55101-X |
| PMID | 21119111 |
| MeSH | Administration, Topical Animals B7-1 Antigen / genetics B7-1 Antigen / metabolism* B7-2 Antigen / genetics B7-2 Antigen / metabolism* Base Sequence Binding Sites / genetics DNA, Complementary / genetics Dendritic Cells / immunology* Dendritic Cells / metabolism* Dermatitis, Contact / genetics Dermatitis, Contact / immunology Dermatitis, Contact / prevention & control Down-Regulation Female In Vitro Techniques Mice Mice, Inbred BALB C Molecular Sequence Data Promoter Regions, Genetic Proto-Oncogene Proteins / antagonists & inhibitors Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism* RNA, Small Interfering / administration & dosage RNA, Small Interfering / genetics Trans-Activators / antagonists & inhibitors Trans-Activators / genetics Trans-Activators / metabolism* Transcription Initiation Site Transcriptional Activation |
| IF | 17.794 |
| 引用数 | 35 |
| WOS 分野 | HEMATOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | CV-1(RCB0160) |