RRC ID 18518
著者 Uto T, Qin GW, Morinaga O, Shoyama Y.
タイトル 17-Hydroxy-jolkinolide B, a diterpenoid from Euphorbia fischeriana, inhibits inflammatory mediators but activates heme oxygenase-1 expression in lipopolysaccharide-stimulated murine macrophages.
ジャーナル Int Immunopharmacol
Abstract Jolkinolides are the main abietane-type diterpenoids isolated from the root of Euphorbia fischeriana Steud. In the present study, we investigated in vitro anti-inflammatory activity of four structural analogs of jolkinolide in lipopolysaccharide (LPS)-stimulated RAW264 macrophages. Among these jolkinolides, 17-hydroxy-jolkinolide B (HJB) exhibited the most potent inhibition of LPS-induced production of inflammatory mediators such as prostaglandin E(2) (PGE(2)), nitric oxide (NO), and pro-inflammatory cytokines [interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α)]. HJB could decrease LPS-induced protein levels of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) and the mRNA expressions of COX-2, iNOS, IL-6, and TNF-α in a concentration-dependent manner. These inhibitory effects were caused by suppression of MAPK phosphorylation and NF-κB activation. Furthermore, we demonstrated that HJB strongly induced heme oxygenase-1 (HO-1) protein and mRNA expressions. These findings suggest that HJB possesses anti-inflammatory actions in macrophages and may provide a potential therapeutic approach for inflammatory disorders.
巻・号 12(1)
ページ 101-9
公開日 2012-1-1
DOI 10.1016/j.intimp.2011.10.020
PII S1567-5769(11)00418-8
PMID 22080918
MeSH Animals Anti-Inflammatory Agents / pharmacology* Cell Line Cells, Cultured Cyclooxygenase 2 / genetics Dinoprostone / metabolism Diterpenes / pharmacology* Down-Regulation Drugs, Chinese Herbal / pharmacology* Euphorbia Female Heme Oxygenase-1 / genetics Heme Oxygenase-1 / metabolism* I-kappa B Kinase / metabolism Interleukin-6 / genetics Lipopolysaccharides Macrophages / drug effects* Macrophages / metabolism Mice Mice, Inbred C57BL Mitogen-Activated Protein Kinases / metabolism NF-kappa B / metabolism Nitric Oxide / metabolism Nitric Oxide Synthase Type II / genetics Plant Roots RNA, Messenger / metabolism Tumor Necrosis Factor-alpha / genetics
IF 3.943
引用数 21
WOS 分野 PHARMACOLOGY & PHARMACY IMMUNOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)