RRC ID 18536
著者 Parajuli LK, Nakajima C, Kulik A, Matsui K, Schneider T, Shigemoto R, Fukazawa Y.
タイトル Quantitative regional and ultrastructural localization of the Ca(v)2.3 subunit of R-type calcium channel in mouse brain.
ジャーナル J Neurosci
Abstract R-type calcium channels (RTCCs) are well known for their role in synaptic plasticity, but little is known about their subcellular distribution across various neuronal compartments. Using subtype-specific antibodies, we characterized the regional and subcellular localization of Ca(v)2.3 in mice and rats at both light and electron microscopic levels. Ca(v)2.3 immunogold particles were found to be predominantly presynaptic in the interpeduncular nucleus, but postsynaptic in other brain regions. Serial section analysis of electron microscopic images from the hippocampal CA1 revealed a higher density of immunogold particles in the dendritic shaft plasma membrane compared with the pyramidal cell somata. However, the labeling densities were not significantly different among the apical, oblique, or basal dendrites. Immunogold particles were also observed over the plasma membrane of dendritic spines, including both synaptic and extrasynaptic sites. Individual spine heads contained <20 immunogold particles, with an average density of ∼260 immunoparticles per μm(3) spine head volume, in accordance with the density of RTCCs estimated using calcium imaging (Sabatini and Svoboda, 2000). The Ca(v)2.3 density was variable among similar-sized spine heads and did not correlate with the density in the parent dendrite, implying that spines are individual calcium compartments operating autonomously from their parent dendrites.
巻・号 32(39)
ページ 13555-67
公開日 2012-9-26
DOI 10.1523/JNEUROSCI.1142-12.2012
PII 32/39/13555
PMID 23015445
PMC PMC6621359
MeSH Analysis of Variance Animals Animals, Newborn Brain / cytology Calcium Channels, R-Type / chemistry Calcium Channels, R-Type / deficiency Calcium Channels, R-Type / metabolism* Calcium Channels, R-Type / ultrastructure* Cation Transport Proteins / chemistry Cation Transport Proteins / deficiency Cation Transport Proteins / metabolism* Cation Transport Proteins / ultrastructure* Cell Membrane / metabolism Cell Membrane / ultrastructure Dendrites / metabolism Dendrites / ultrastructure Dendritic Spines / metabolism Dendritic Spines / ultrastructure Epitopes / metabolism Female Guinea Pigs Imaging, Three-Dimensional Immunohistochemistry Male Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Immunoelectron Neurons / metabolism* Neurons / ultrastructure* Peptides / metabolism Post-Synaptic Density / metabolism Post-Synaptic Density / ultrastructure Rats Statistics as Topic Statistics, Nonparametric Subcellular Fractions / metabolism Subcellular Fractions / ultrastructure
IF 5.674
引用数 46
WOS 分野 NEUROSCIENCES
リソース情報
実験動物マウス RBRC04885