論文 - 詳細
RRC ID | 18564 |
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著者 | Sawai H, Domae N. |
タイトル | Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells. |
ジャーナル | Biochem Biophys Res Commun |
Abstract |
Apoptotic cell death eventually results in secondary necrotic cell death, whereas caspase-independent primary necrotic cell death has been reported and its mechanism involving RIP1 and RIP3 has been recently elucidated. Dual staining with fluorescent Annexin V and propidium iodide (PI) has been used to discriminate apoptotic and necrotic cell death, in which Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis. Here we demonstrate that primary necrotic cells unexpectedly show Annexin V-positive/PI-negative staining before they become PI-positive, and that primary necrotic and apoptotic Annexin V-positive/PI-negative cells can be discriminated by necrostatin-1, an inhibitor of primary necrosis by inhibition of RIP1. |
巻・号 | 411(3) |
ページ | 569-73 |
公開日 | 2011-8-5 |
DOI | 10.1016/j.bbrc.2011.06.186 |
PII | S0006-291X(11)01199-5 |
PMID | 21763280 |
MeSH | Annexin A5 / analysis Annexin A5 / metabolism* Apoptosis / drug effects* Caspases / metabolism Coloring Agents / chemistry* Humans Imidazoles / pharmacology* Indoles / pharmacology* Necrosis Nuclear Pore Complex Proteins / antagonists & inhibitors* Propidium / chemistry* RNA-Binding Proteins / antagonists & inhibitors* Staining and Labeling U937 Cells |
IF | 2.985 |
引用数 | 94 |
WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | U-937 DE-4(RCB0435) |