論文 - 詳細
| RRC ID | 18600 |
|---|---|
| 著者 | Seike T, Yamagishi Y, Iio H, Nakamura T, Shimoda C. |
| タイトル | Remarkably simple sequence requirement of the M-factor pheromone of Schizosaccharomyces pombe. |
| ジャーナル | Genetics |
| Abstract |
The mating reaction is triggered by specific pheromones in a wide variety of organisms. Small peptides are used as mating pheromones in yeasts and fungi. In the fission yeast Schizosaccharomyces pombe, M-factor is a C terminally farnesylated nonapeptide secreted from M-cells, and its counterpart, P-factor, is a simple peptide composed of 23 amino acids. The primary structure requirements for the biological activity of pheromone peptides remain to be elucidated. Here, we conducted comprehensive substitution of each of the amino acids in M-factor peptide and inspected the mating ability of these missense mutants. Thirty-five sterile mutants were found among an array of 152 mutants with single amino acid substitutions. Mapping of the mutation sites clearly indicated that the sterile mutants were associated exclusively with four amino acid residues (VPYM) in the carboxyl-terminal half. In contrast, the substitution of four amino-terminal residues (YTPK) with any amino acid had no or only a slightly deleterious effect on mating. Furthermore, deletion of the three N-terminal residues caused no sterility, although truncation of a fourth residue had a marked effect. We conclude that a farnesylated hexapeptide (KVPYMC(Far)-OCH(3)) is the minimal M-factor that retains pheromone activity. At least 15 nonfunctional peptides were found to be secreted, suggesting that these mutant M-factor peptides are no longer recognized by the cognate receptor. |
| 巻・号 | 191(3) |
| ページ | 815-25 |
| 公開日 | 2012-7-1 |
| DOI | 10.1534/genetics.112.140483 |
| PII | genetics.112.140483 |
| PMID | 22542965 |
| PMC | PMC3389977 |
| MeSH | Amino Acid Sequence Cell Aggregation Conserved Sequence DNA-Binding Proteins Molecular Sequence Data Mutagenesis Mutation, Missense Peptides / chemistry* Peptides / genetics Peptides / metabolism* Pheromones / chemistry* Pheromones / genetics Pheromones / metabolism* Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / chemistry* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Deletion Structure-Activity Relationship Zygote / metabolism |
| IF | 4.015 |
| 引用数 | 10 |
| WOS 分野 | GENETICS & HEREDITY |
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オルトメトリクス指標項目
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| 最多言及媒体 | Blogs |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 5.0 |
| リソース情報 | |
| 一般微生物 | JCM 8262 JCM 8274 JCM 21876 JCM 21877 |
| 酵母 | |