RRC ID 18600
著者 Seike T, Yamagishi Y, Iio H, Nakamura T, Shimoda C.
タイトル Remarkably simple sequence requirement of the M-factor pheromone of Schizosaccharomyces pombe.
ジャーナル Genetics
Abstract The mating reaction is triggered by specific pheromones in a wide variety of organisms. Small peptides are used as mating pheromones in yeasts and fungi. In the fission yeast Schizosaccharomyces pombe, M-factor is a C terminally farnesylated nonapeptide secreted from M-cells, and its counterpart, P-factor, is a simple peptide composed of 23 amino acids. The primary structure requirements for the biological activity of pheromone peptides remain to be elucidated. Here, we conducted comprehensive substitution of each of the amino acids in M-factor peptide and inspected the mating ability of these missense mutants. Thirty-five sterile mutants were found among an array of 152 mutants with single amino acid substitutions. Mapping of the mutation sites clearly indicated that the sterile mutants were associated exclusively with four amino acid residues (VPYM) in the carboxyl-terminal half. In contrast, the substitution of four amino-terminal residues (YTPK) with any amino acid had no or only a slightly deleterious effect on mating. Furthermore, deletion of the three N-terminal residues caused no sterility, although truncation of a fourth residue had a marked effect. We conclude that a farnesylated hexapeptide (KVPYMC(Far)-OCH(3)) is the minimal M-factor that retains pheromone activity. At least 15 nonfunctional peptides were found to be secreted, suggesting that these mutant M-factor peptides are no longer recognized by the cognate receptor.
巻・号 191(3)
ページ 815-25
公開日 2012-7-1
DOI 10.1534/genetics.112.140483
PII genetics.112.140483
PMID 22542965
PMC PMC3389977
MeSH Amino Acid Sequence Cell Aggregation Conserved Sequence DNA-Binding Proteins Molecular Sequence Data Mutagenesis Mutation, Missense Peptides / chemistry* Peptides / genetics Peptides / metabolism* Pheromones / chemistry* Pheromones / genetics Pheromones / metabolism* Schizosaccharomyces / cytology Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / chemistry* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Deletion Structure-Activity Relationship Zygote / metabolism
IF 4.015
引用数 10
WOS 分野 GENETICS & HEREDITY
リソース情報
一般微生物 JCM 8262 JCM 8274 JCM 21876 JCM 21877
酵母