RRC ID 1867
Author Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A.
Title The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis.
Journal Plant Cell
Abstract The outermost epidermal cell wall is specialized to withstand pathogens and natural stresses, and lipid-based cuticular polymers are the major barrier against incursions. The Arabidopsis thaliana mutant bodyguard (bdg), which exhibits defects characteristic of the loss of cuticle structure not attributable to a lack of typical cutin monomers, unexpectedly accumulates significantly more cell wall-bound lipids and epicuticular waxes than wild-type plants. Pleiotropic effects of the bdg mutation on growth, viability, and cell differentiation are also observed. BDG encodes a member of the alpha/beta-hydrolase fold protein superfamily and is expressed exclusively in epidermal cells. Using Strep-tag epitope-tagged BDG for mutant complementation and immunolocalization, we show that BDG is a polarly localized protein that accumulates in the outermost cell wall in the epidermis. With regard to the appearance and structure of the cuticle, the phenotype conferred by bdg is reminiscent of that of transgenic Arabidopsis plants that express an extracellular fungal cutinase, suggesting that bdg may be incapable of completing the polymerization of carboxylic esters in the cuticular layer of the cell wall or the cuticle proper. We propose that BDG codes for an extracellular synthase responsible for the formation of cuticle. The alternative hypothesis proposes that BDG controls the proliferation/differentiation status of the epidermis via an unknown mechanism.
Volume 18(2)
Pages 321-39
Published 2006-2
DOI 10.1105/tpc.105.036079
PII tpc.105.036079
PMID 16415209
PMC PMC1356542
MeSH Amino Acid Sequence Arabidopsis / anatomy & histology Arabidopsis / growth & development* Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cloning, Molecular DNA Transposable Elements / genetics Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genetic Complementation Test Hydrolases / chemistry Lipids / analysis Molecular Sequence Data Morphogenesis* Organ Specificity Phenotype Plant Epidermis / growth & development* Plant Epidermis / metabolism* Plant Epidermis / ultrastructure Plant Leaves / anatomy & histology Plant Leaves / ultrastructure Plants, Genetically Modified / metabolism Protein Carbonylation Protein Folding Protein Transport RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Alignment Waxes / metabolism
IF 8.228
Times Cited 136
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda12166 pda10970