Reference - Detail
RRC ID | 18705 |
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Author | Itoh T, Takeda S, Akao Y. |
Title | MicroRNA-208 modulates BMP-2-stimulated mouse preosteoblast differentiation by directly targeting V-ets erythroblastosis virus E26 oncogene homolog 1. |
Journal | J Biol Chem |
Abstract |
MicroRNAs (miRs) represent a class of endogenous approximately 18-25 nucleotide RNAs that regulate gene expression through translational repression by binding to a target mRNA. These miRs regulate several biological functions, such as cell growth, cell differentiation, carcinogenesis, and so on. In a previous report, we have indicated that miR-141 and -200a act as preosteoblast differentiation modulators. In the present study, using microRNA array and in silico analyses, we found that miR-208 is closely involved in preosteoblast differentiation by partially regulating the expression of Ets1 (V-ets erythroblastosis virus E26 oncogene homolog 1), which transactivates osteopontin, runt-related transcription factor 2, parathyroid hormone-related protein, and type I procollagen. Furthermore, the enforced expression of mature miR-208 in murine preosteoblast in MC3T3-E1 cells or primary osteoblast cells remarkably attenuated BMP-2-induced preosteoblast differentiation. In addition, we determined that Ets1 is a target gene of miR-208 by using a sensor luciferase reporter assay. Taken together, these results suggest that the down-regulation of miR-208 in BMP-2-stimulated osteoblast differentiation is an important part of the regulatory machinery involved in early osteogenesis. |
Volume | 285(36) |
Pages | 27745-52 |
Published | 2010-9-3 |
DOI | 10.1074/jbc.M110.105080 |
PII | S0021-9258(19)89106-8 |
PMID | 20576608 |
PMC | PMC2934642 |
MeSH | 3T3 Cells Animals Base Sequence Blotting, Western Bone Morphogenetic Protein 2 / pharmacology* Cell Differentiation / drug effects* Cell Differentiation / genetics Computational Biology Enzyme Activation / drug effects Enzyme Activation / genetics Gene Expression Profiling Genes, Reporter / genetics Humans Luciferases / genetics Mice MicroRNAs / genetics* Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Oligonucleotide Array Sequence Analysis Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / enzymology Osteoblasts / metabolism* Osteogenesis / drug effects Osteogenesis / genetics Proto-Oncogene Protein c-ets-1 / deficiency Proto-Oncogene Protein c-ets-1 / genetics* Rats |
IF | 4.238 |
Times Cited | 54 |
WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
Resource | |
Human and Animal Cells | MC3T3-E1(RCB1126) |