| RRC ID |
18720
|
| 著者 |
Kato Y, Koide N, Komatsu T, Tumurkhuu G, Dagvadorj J, Kato K, Yokochi T.
|
| タイトル |
Metformin attenuates production of nitric oxide in response to lipopolysaccharide by inhibiting MyD88-independent pathway.
|
| ジャーナル |
Horm Metab Res
|
| Abstract |
Metformin is reported to ameliorate inflammation in diabetic patients. The effect of metformin on lipopolysaccharide-induced nitric oxide production was studied by using RAW 264.7 macrophage-like cells. The action of metformin was analyzed by dividing lipopolysaccharide signaling into the MyD88-dependent and -independent pathways. Metformin significantly reduced the expression of an inducible type of nitric oxide synthase and inhibited lipopolysaccharide-induced nitric oxide production. On the other hand, metformin did not inhibit lipopolysaccharide-induced tumor necrosis factor-alpha production. The expression levels of interferon-beta protein and mRNA, which is a key molecule in MyD88-independent pathway, were significantly inhibited by metformin. Compound C, a specific AMP-activated protein kinase inhibitor, did not affect the inhibitory action of metformin. Metformin was suggested to inhibit lipopolysaccharide-induced nitric oxide production via inhibition of interferon-beta production in MyD88-independent pathway. Metformin might exhibit an anti- inflammatory action on diabetic complications as well as the antidiabetic action.
|
| 巻・号 |
42(9)
|
| ページ |
632-6
|
| 公開日 |
2010-8-1
|
| DOI |
10.1055/s-0030-1255033
|
| PMID |
20560107
|
| MeSH |
AMP-Activated Protein Kinases / antagonists & inhibitors
Animals
Cell Line
Gene Expression Regulation / drug effects
Interferon-beta / genetics
Interferon-beta / metabolism
Lipopolysaccharides / pharmacology*
Macrophages / drug effects*
Macrophages / enzymology
Macrophages / metabolism*
Metformin / pharmacology*
Mice
Mice, Inbred C57BL
Myeloid Differentiation Factor 88 / metabolism
Nitric Oxide / biosynthesis*
Poly I-C / pharmacology
Protein Kinase Inhibitors / pharmacology
RNA, Messenger / genetics
RNA, Messenger / metabolism
Signal Transduction / drug effects*
Tumor Necrosis Factor-alpha / biosynthesis
|
| IF |
2.562
|
| 引用数 |
17
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
