論文 - 詳細
| RRC ID | 18720 |
|---|---|
| 著者 | Kato Y, Koide N, Komatsu T, Tumurkhuu G, Dagvadorj J, Kato K, Yokochi T. |
| タイトル | Metformin attenuates production of nitric oxide in response to lipopolysaccharide by inhibiting MyD88-independent pathway. |
| ジャーナル | Horm Metab Res |
| Abstract |
Metformin is reported to ameliorate inflammation in diabetic patients. The effect of metformin on lipopolysaccharide-induced nitric oxide production was studied by using RAW 264.7 macrophage-like cells. The action of metformin was analyzed by dividing lipopolysaccharide signaling into the MyD88-dependent and -independent pathways. Metformin significantly reduced the expression of an inducible type of nitric oxide synthase and inhibited lipopolysaccharide-induced nitric oxide production. On the other hand, metformin did not inhibit lipopolysaccharide-induced tumor necrosis factor-alpha production. The expression levels of interferon-beta protein and mRNA, which is a key molecule in MyD88-independent pathway, were significantly inhibited by metformin. Compound C, a specific AMP-activated protein kinase inhibitor, did not affect the inhibitory action of metformin. Metformin was suggested to inhibit lipopolysaccharide-induced nitric oxide production via inhibition of interferon-beta production in MyD88-independent pathway. Metformin might exhibit an anti- inflammatory action on diabetic complications as well as the antidiabetic action. |
| 巻・号 | 42(9) |
| ページ | 632-6 |
| 公開日 | 2010-8-1 |
| DOI | 10.1055/s-0030-1255033 |
| PMID | 20560107 |
| MeSH | AMP-Activated Protein Kinases / antagonists & inhibitors Animals Cell Line Gene Expression Regulation / drug effects Interferon-beta / genetics Interferon-beta / metabolism Lipopolysaccharides / pharmacology* Macrophages / drug effects* Macrophages / enzymology Macrophages / metabolism* Metformin / pharmacology* Mice Mice, Inbred C57BL Myeloid Differentiation Factor 88 / metabolism Nitric Oxide / biosynthesis* Poly I-C / pharmacology Protein Kinase Inhibitors / pharmacology RNA, Messenger / genetics RNA, Messenger / metabolism Signal Transduction / drug effects* Tumor Necrosis Factor-alpha / biosynthesis |
| IF | 2.562 |
| 引用数 | 17 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |