Reference - Detail
|Author||Noman AS, Koide N, Iftakhar-E-Khuda I, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T.|
|Title||Retinoblastoma protein-interacting zinc finger 1 (RIZ1) participates in RANKL-induced osteoclast formation via regulation of NFATc1 expression.|
The role of retinoblastoma protein-interacting zinc finger 1 (RIZ1) in receptor activator of NF-kappaB ligand (RANKL)-induced osteoclast formation was examined in mouse RAW 264.7 macrophage-like cells. The expression of RIZ1 was significantly augmented by RANKL-treated cells. Silencing of RIZ1 with the siRNA significantly reduced the appearance of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells as osteoclasts in RANKL-treated cells. The expression of nuclear factor of activated T cell 1 (NFATc1) as the terminal transcription factor of osteoclast formation was prevented by RIZ1 siRNA. It was suggested that that RIZ1 might participate in RANKL-induced osteoclast formation through the regulation of NFATc1 expression.
|MeSH||Acid Phosphatase / biosynthesis Animals Antigens, Differentiation / biosynthesis Bone Resorption Cell Line Gene Expression Regulation Histone-Lysine N-Methyltransferase / genetics Histone-Lysine N-Methyltransferase / immunology Histone-Lysine N-Methyltransferase / metabolism* Isoenzymes / biosynthesis Macrophages / immunology Macrophages / metabolism* Macrophages / pathology Mice NFATC Transcription Factors / biosynthesis* NFATC Transcription Factors / genetics Neoplasms / metabolism* Neoplasms / pathology Neoplasms / physiopathology Osteoclasts / immunology Osteoclasts / metabolism* Osteoclasts / pathology RANK Ligand / metabolism RNA, Small Interfering / genetics Signal Transduction / genetics Signal Transduction / immunology Tartrate-Resistant Acid Phosphatase Transcription Factors / genetics Transcription Factors / immunology Transcription Factors / metabolism*|
|Human and Animal Cells||RAW 264 (RCB0535)|