RRC ID 1888
Author Fujibe T, Saji H, Watahiki MK, Yamamoto KT.
Title Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) gene of Arabidopsis causes weak rcd1 phenotype with compromised oxidative-stress responses.
Journal Biosci. Biotechnol. Biochem.
Abstract rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.
Volume 70(8)
Pages 1827-31
Published 2006-8
DOI 10.1271/bbb.50673
PII JST.JSTAGE/bbb/50673
PMID 16926493
MeSH Arabidopsis / drug effects Arabidopsis / genetics* Arabidopsis Proteins / drug effects Arabidopsis Proteins / genetics* Gene Expression Profiling* Green Fluorescent Proteins / genetics Mutation Nuclear Proteins / drug effects Nuclear Proteins / genetics* Oxidative Stress / physiology* Ozone / pharmacology Phenotype RNA, Messenger / drug effects RNA, Messenger / genetics Recombinant Fusion Proteins / genetics Reverse Transcriptase Polymerase Chain Reaction / methods Seeds / drug effects Seeds / genetics
IF 1.255
Times Cited 10
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda04792 pda08945