論文 - 詳細
| RRC ID | 1888 |
|---|---|
| 著者 | Fujibe T, Saji H, Watahiki MK, Yamamoto KT. |
| タイトル | Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) gene of Arabidopsis causes weak rcd1 phenotype with compromised oxidative-stress responses. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region. |
| 巻・号 | 70(8) |
| ページ | 1827-31 |
| 公開日 | 2006-8-1 |
| DOI | 10.1271/bbb.50673 |
| PII | JST.JSTAGE/bbb/50673 |
| PMID | 16926493 |
| MeSH | Arabidopsis / drug effects Arabidopsis / genetics* Arabidopsis Proteins / drug effects Arabidopsis Proteins / genetics* Gene Expression Profiling* Green Fluorescent Proteins / genetics Mutation Nuclear Proteins / drug effects Nuclear Proteins / genetics* Oxidative Stress / physiology* Ozone / pharmacology Phenotype RNA, Messenger / drug effects RNA, Messenger / genetics Recombinant Fusion Proteins / genetics Reverse Transcriptase Polymerase Chain Reaction / methods Seeds / drug effects Seeds / genetics |
| IF | 1.516 |
| 引用数 | 13 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda04792 pda08945 |