| RRC ID |
1888
|
| Author |
Fujibe T, Saji H, Watahiki MK, Yamamoto KT.
|
| Title |
Overexpression of the RADICAL-INDUCED CELL DEATH1 (RCD1) gene of Arabidopsis causes weak rcd1 phenotype with compromised oxidative-stress responses.
|
| Journal |
Biosci Biotechnol Biochem
|
| Abstract |
rcd1 is a mutant of Arabidopsis thaliana that is more resistant to methyl viologen, but more sensitive to ozone than the wild type. rcd1-2 is caused by a single nucleotide substitution that results in a premature stop codon at Trp-332. The rcd1-2 mRNA level does not change significantly with the mutation. Since overexpression of rcd1-1 cDNA has been shown to bring about an rcd1-like phenotype, we created and examined the overexpression lines of RCD1 by the use of the cauliflower mosaic virus 35S promoter. The transgenic lines exhibited a weak rcd1-like phenotype, although no resistance to methyl viologen was observed. Further, they fully complemented the aberrant rcd1-2 phenotype. Subcellular localization of RCD1 was examined by transiently expressing green fluorescent protein (GFP) fused with RCD1 in onion epidermal cells. GFP signals are observed as aggregated foci in the inner nuclear matrix-like region.
|
| Volume |
70(8)
|
| Pages |
1827-31
|
| Published |
2006-8-1
|
| DOI |
10.1271/bbb.50673
|
| PII |
JST.JSTAGE/bbb/50673
|
| PMID |
16926493
|
| MeSH |
Arabidopsis / drug effects
Arabidopsis / genetics*
Arabidopsis Proteins / drug effects
Arabidopsis Proteins / genetics*
Gene Expression Profiling*
Green Fluorescent Proteins / genetics
Mutation
Nuclear Proteins / drug effects
Nuclear Proteins / genetics*
Oxidative Stress / physiology*
Ozone / pharmacology
Phenotype
RNA, Messenger / drug effects
RNA, Messenger / genetics
Recombinant Fusion Proteins / genetics
Reverse Transcriptase Polymerase Chain Reaction / methods
Seeds / drug effects
Seeds / genetics
|
| IF |
1.516
|
| Times Cited |
13
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
CHEMISTRY, APPLIED
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda04792
pda08945 |
