RRC ID 1894
Author Mitsuda N, Hiratsu K, Todaka D, Nakashima K, Yamaguchi-Shinozaki K, Ohme-Takagi M.
Title Efficient production of male and female sterile plants by expression of a chimeric repressor in Arabidopsis and rice.
Journal Plant Biotechnol. J.
Abstract Male and female sterile plants are particularly useful for the effective production of commercial hybrid plants and for preventing the diffusion of seeds or pollen grains of genetically modified plants in the open field. In an attempt to create several types of sterile plant by genetic manipulation, we applied our Chimeric REpressor Gene-Silencing Technology (CRES-T) to four transcription factors, namely APETALA3, AGAMOUS, LEAFY and AtMYB26, involved in the regulation of petal and stamen identity, stamen and carpel identity, floral meristem identity and anther dehiscence, respectively, in Arabidopsis. Transgenic plants expressing each chimeric repressor exhibited, at high frequency, a sterile phenotype that resembled the loss-of-function phenotype of each corresponding gene. Furthermore, in the monocotyledonous crop plant 'rice', expression of the chimeric repressor derived from SUPERWOMAN1, the rice orthologue of APETALA3, resulted in the male sterile phenotype with high efficiency. Our results indicate that CRES-T provides a powerful tool for controlling the fertility of both monocots and dicots by exploiting transcription factors that are strongly conserved amongst plants.
Volume 4(3)
Pages 325-32
Published 2006-5
DOI 10.1111/j.1467-7652.2006.00184.x
PII PBI184
PMID 17147638
MeSH AGAMOUS Protein, Arabidopsis / genetics AGAMOUS Protein, Arabidopsis / metabolism Arabidopsis / genetics* Arabidopsis / metabolism Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Gene Expression Regulation, Plant Gene Silencing* Genetic Engineering / methods MADS Domain Proteins / genetics MADS Domain Proteins / metabolism Mutation Oryza / genetics* Oryza / metabolism Phenotype Plant Infertility / physiology* Plant Proteins / genetics* Plant Proteins / metabolism Plants, Genetically Modified / anatomy & histology Plants, Genetically Modified / metabolism Plants, Genetically Modified / physiology Recombinant Fusion Proteins / metabolism Repressor Proteins / genetics* Repressor Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism
IF 6.305
Times Cited 81
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY PLANT SCIENCES
Resource
Arabidopsis / Cultured plant cells, genes pda06813