論文 - 詳細
| RRC ID | 18987 |
|---|---|
| 著者 | Feng GG, Li C, Huang L, Tsunekawa K, Sato Y, Fujiwara Y, Komatsu T, Honda T, Fan JH, Goto H, Koide T, Hasegawa T, Ishikawa N. |
| タイトル | Naofen, a novel WD40-repeat protein, mediates spontaneous and tumor necrosis factor-induced apoptosis. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Naofen has recently been identified from the rat brain/spinal cord cDNA library as a substance reactive against an anti-shigatoxin (Stx)-2 antibody. Naofen mRNA is composed of 4620 nucleotides and encodes 1170 amino acids. Naofen contains four WD-repeat domains in its N-terminus and is ubiquitously distributed in many tissues of the rat. Tumor necrosis factor (TNF)-alpha enhanced the expression of naofen mRNA in HEK293 cells in a dose-dependent manner. Furthermore, naofen siRNA, which predominantly knocked down the expression of naofen mRNA, significantly reduced both TNF-alpha-induced caspase-3 activation and apoptosis in HEK293 cells. Overexpression of naofen in HEK293 cells (FLAG-NF) spontaneously induced caspase -3 activation and apoptosis, and showed extremely high susceptibility to TNF-alpha-induced apoptosis. These results indicated that naofen may function as a novel modulator activating caspase-3, and promoting TNF-alpha-stimulated apoptosis. |
| 巻・号 | 394(1) |
| ページ | 153-7 |
| 公開日 | 2010-3-26 |
| DOI | 10.1016/j.bbrc.2010.02.133 |
| PII | S0006-291X(10)00367-0 |
| PMID | 20193664 |
| MeSH | Animals Apoptosis* Apoptosis Regulatory Proteins / genetics Apoptosis Regulatory Proteins / metabolism* Caspase 3 / biosynthesis Cell Line Enzyme Activation Humans Proteins / genetics Proteins / metabolism* RNA, Messenger / metabolism Rats Rats, Wistar Tissue Distribution Tumor Necrosis Factor-alpha / pharmacology Tumor Necrosis Factor-alpha / physiology |
| IF | 2.985 |
| 引用数 | 10 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | 293(RCB1637) |