RRC ID 18988
Author Mikami Y, Ishii Y, Watanabe N, Shirakawa T, Suzuki S, Irie S, Isokawa K, Honda MJ.
Title CD271/p75(NTR) inhibits the differentiation of mesenchymal stem cells into osteogenic, adipogenic, chondrogenic, and myogenic lineages.
Journal Stem Cells Dev.
Abstract We describe a novel role for CD271 in the differentiation of mesenchymal stem cells (MSCs), including deciduous dental pulp stem cells (DDPSCs) and murine multipotent MSCs (C3H10T1/2 cells). The CD271(+) subpopulation of deciduous dental pulp cells (CD271(+)/DDPSCs) and the forced expression of CD271 in C3H10T1/2 (10T271) were analyzed by fluorescence-activated cell sorting. CD271 expression was detected in DDPSCs that expressed both CD44 and CD90, simultaneously, and the clonogenic capacity of the CD271(+)/DDPSCs was higher than that of the CD271(-)/DDPSCs that expressed both CD44 and CD90. Further, the differentiation of CD271(+)/DDPSCs into osteoblasts and adipocytes was inhibited although CD271(-)/DDPSCs were capable of differentiating into osteoblasts and adipocytes. CD271 was overexpressed in C3H10T1/2 cells, which have the potential to differentiate into osteoblasts, adipocytes, chondrocytes, and myocytes. CD271 inhibited the differentiation of C3H10T1/2 cells into any of these lineages. These results indicate a role for CD271 in inhibiting the differentiation of MSCs.
Volume 20(5)
Pages 901-13
Published 2011-5
DOI 10.1089/scd.2010.0299
PMID 21142793
MeSH Adipocytes / cytology Adipocytes / metabolism Animals Cell Differentiation Cell Lineage / genetics Cell Proliferation Cells, Cultured Chondrocytes / cytology Chondrocytes / metabolism Dental Pulp / cytology* Dental Pulp / metabolism Flow Cytometry Gene Expression Regulation, Developmental Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / metabolism Mice Multipotent Stem Cells / cytology* Multipotent Stem Cells / metabolism Muscle Cells / cytology Muscle Cells / metabolism Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism* Osteoblasts / cytology Osteoblasts / metabolism Plasmids RNA, Messenger / analysis RNA, Messenger / biosynthesis Receptors, Nerve Growth Factor / genetics Receptors, Nerve Growth Factor / metabolism* Reverse Transcriptase Polymerase Chain Reaction Tooth, Deciduous / cytology Tooth, Deciduous / metabolism Transfection
IF 3.315
Times Cited 38
Human and Animal Cells 10T1/2 (RCB0247)