RRC ID |
18988
|
著者 |
Mikami Y, Ishii Y, Watanabe N, Shirakawa T, Suzuki S, Irie S, Isokawa K, Honda MJ.
|
タイトル |
CD271/p75(NTR) inhibits the differentiation of mesenchymal stem cells into osteogenic, adipogenic, chondrogenic, and myogenic lineages.
|
ジャーナル |
Stem Cells Dev
|
Abstract |
We describe a novel role for CD271 in the differentiation of mesenchymal stem cells (MSCs), including deciduous dental pulp stem cells (DDPSCs) and murine multipotent MSCs (C3H10T1/2 cells). The CD271(+) subpopulation of deciduous dental pulp cells (CD271(+)/DDPSCs) and the forced expression of CD271 in C3H10T1/2 (10T271) were analyzed by fluorescence-activated cell sorting. CD271 expression was detected in DDPSCs that expressed both CD44 and CD90, simultaneously, and the clonogenic capacity of the CD271(+)/DDPSCs was higher than that of the CD271(-)/DDPSCs that expressed both CD44 and CD90. Further, the differentiation of CD271(+)/DDPSCs into osteoblasts and adipocytes was inhibited although CD271(-)/DDPSCs were capable of differentiating into osteoblasts and adipocytes. CD271 was overexpressed in C3H10T1/2 cells, which have the potential to differentiate into osteoblasts, adipocytes, chondrocytes, and myocytes. CD271 inhibited the differentiation of C3H10T1/2 cells into any of these lineages. These results indicate a role for CD271 in inhibiting the differentiation of MSCs.
|
巻・号 |
20(5)
|
ページ |
901-13
|
公開日 |
2011-5-1
|
DOI |
10.1089/scd.2010.0299
|
PMID |
21142793
|
MeSH |
Adipocytes / cytology
Adipocytes / metabolism
Animals
Cell Differentiation
Cell Lineage / genetics
Cell Proliferation
Cells, Cultured
Chondrocytes / cytology
Chondrocytes / metabolism
Dental Pulp / cytology*
Dental Pulp / metabolism
Flow Cytometry
Gene Expression Regulation, Developmental
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism
Mice
Multipotent Stem Cells / cytology*
Multipotent Stem Cells / metabolism
Muscle Cells / cytology
Muscle Cells / metabolism
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / metabolism*
Osteoblasts / cytology
Osteoblasts / metabolism
Plasmids
RNA, Messenger / analysis
RNA, Messenger / biosynthesis
Receptors, Nerve Growth Factor / genetics
Receptors, Nerve Growth Factor / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Tooth, Deciduous / cytology
Tooth, Deciduous / metabolism
Transfection
|
IF |
3.153
|
引用数 |
51
|
WOS 分野
|
TRANSPLANTATION
MEDICINE, RESEARCH & EXPERIMENTAL
HEMATOLOGY
CELL & TISSUE ENGINEERING
|
リソース情報 |
ヒト・動物細胞 |
10T1/2(RCB0247) |