Reference - Detail
|Author||Ishibashi N, Himeno K, Fujita K, Masuda Y, Perez RH, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto K.|
|Title||Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish.|
|Journal||Biosci Biotechnol Biochem|
Enterocins NKR-5-3A, B, C, and D were purified from the culture supernatant of Enterococcus faecium NKR-5-3 and characterized. Among the four purified peptides, enterocin NKR-5-3A (5242.3 Da) was identical to brochocin A, produced by Brochothrix campestris ATCC 43754, in mature peptides, and its putative synergistic peptide, enterocin NKR-5-3Z, was found to be encoded in ent53Z downstream of ent53A, encoding enterocin NKR-5-3A. Enterocin NKR-5-3B (6316.4 Da) showed a broad antimicrobial spectrum, and enterocin NKR-5-3C (4512.8 Da) showed high activity against Listeria. Enterocin NKR-5-3D (2843.5 Da), showing high homology to an inducing peptide produced by Lactobacillus sakei 5, induced the production of the enterocins. The enterocins showed different antimicrobial spectra and intensities. E. faecium NKR-5-3 concomitantly produced enterocins NKR-5-3A, B, C, and D which probably belong to different classes of bacteriocins. Furthermore, NKR-5-3 production was induced by enterocin NKR-5-3D.
|MeSH||Amino Acid Sequence Animals Bacteriocins / biosynthesis Bacteriocins / genetics Bacteriocins / isolation & purification* Base Sequence Chromatography, Reverse-Phase Enterococcus faecium / genetics Enterococcus faecium / metabolism* Fermentation Fishes / microbiology* Lactobacillus / drug effects Lactobacillus / metabolism Microbial Sensitivity Tests Microbial Viability / drug effects Molecular Sequence Data Peptides / genetics Peptides / isolation & purification* Peptides / metabolism Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Sequence Analysis, DNA|
|WOS Category||FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY|
|General Microbes||JCM 1157 JCM 1465 JCM 2257 JCM 5803 JCM 5885|