RRC ID 19124
Author Ishibashi N, Himeno K, Fujita K, Masuda Y, Perez RH, Zendo T, Wilaipun P, Leelawatcharamas V, Nakayama J, Sonomoto K.
Title Purification and characterization of multiple bacteriocins and an inducing peptide produced by Enterococcus faecium NKR-5-3 from Thai fermented fish.
Journal Biosci. Biotechnol. Biochem.
Abstract Enterocins NKR-5-3A, B, C, and D were purified from the culture supernatant of Enterococcus faecium NKR-5-3 and characterized. Among the four purified peptides, enterocin NKR-5-3A (5242.3 Da) was identical to brochocin A, produced by Brochothrix campestris ATCC 43754, in mature peptides, and its putative synergistic peptide, enterocin NKR-5-3Z, was found to be encoded in ent53Z downstream of ent53A, encoding enterocin NKR-5-3A. Enterocin NKR-5-3B (6316.4 Da) showed a broad antimicrobial spectrum, and enterocin NKR-5-3C (4512.8 Da) showed high activity against Listeria. Enterocin NKR-5-3D (2843.5 Da), showing high homology to an inducing peptide produced by Lactobacillus sakei 5, induced the production of the enterocins. The enterocins showed different antimicrobial spectra and intensities. E. faecium NKR-5-3 concomitantly produced enterocins NKR-5-3A, B, C, and D which probably belong to different classes of bacteriocins. Furthermore, NKR-5-3 production was induced by enterocin NKR-5-3D.
Volume 76(5)
Pages 947-53
Published 2012
DOI 10.1271/bbb.110972
PII DN/JST.JSTAGE/bbb/110972
PMID 22738965
MeSH Amino Acid Sequence Animals Bacteriocins / biosynthesis Bacteriocins / genetics Bacteriocins / isolation & purification* Base Sequence Chromatography, Reverse-Phase Enterococcus faecium / genetics Enterococcus faecium / metabolism* Fermentation Fishes / microbiology* Lactobacillus / drug effects Lactobacillus / metabolism Microbial Sensitivity Tests Microbial Viability / drug effects Molecular Sequence Data Peptides / genetics Peptides / isolation & purification* Peptides / metabolism Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / isolation & purification Sequence Analysis, DNA
IF 1.297
Times Cited 25
WOS Category FOOD SCIENCE & TECHNOLOGY CHEMISTRY, APPLIED BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
General Microbes JCM 1157 JCM 1465 JCM 2257 JCM 5803 JCM 5885