Reference - Detail
| RRC ID | 19145 |
|---|---|
| Author | Sugiyama D, Kulkeaw K, Mizuochi C. |
| Title | TGF-beta-1 up-regulates extra-cellular matrix production in mouse hepatoblasts. |
| Journal | Mech Dev |
| Abstract |
Fetal liver is the major embryonic hematopoietic organ and is extrinsically colonized by circulating hematopoietic stem cells (HSCs). Integrin beta-1 expression on HSCs is crucial for colonization, suggesting that interaction of Integrin beta-1 with extra-cellular matrix (ECM) factors promotes HSC adherence to fetal liver. However, little is known about how ECM production is regulated in fetal liver. Here we used flow cytometry to sort fetal liver compartments and detected ECM gene and protein expression predominantly in sorted hepatoblasts. mRNA and protein analysis suggested that TGF-beta-1 expressed by hepatoblasts, sinusoid endothelial cells and hematopoietic cells, binds to the TGF-beta receptor type-2 expressed on hepatoblasts to stimulate ECM production. Intra-cardiac injection of TGF-inhibitors into mouse embryos dramatically decreased fetal liver ECM gene expression. Taken together, our observations suggest that hepatoblasts predominantly produce ECM factors under control of TGF-beta-1 in fetal liver. |
| Volume | 130(2-3) |
| Pages | 195-206 |
| Published | 2013-2-1 |
| DOI | 10.1016/j.mod.2012.09.003 |
| PII | S0925-4773(12)00088-3 |
| PMID | 23041440 |
| MeSH | Animals Antigens, CD / metabolism Cells, Cultured Embryo Culture Techniques Extracellular Matrix / genetics Extracellular Matrix / metabolism* Extracellular Matrix Proteins / genetics Extracellular Matrix Proteins / metabolism Gene Expression Gene Expression Regulation, Developmental Hematopoietic Stem Cells / metabolism Integrins / metabolism Liver / cytology* Liver / embryology Liver / metabolism Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Transgenic Proto-Oncogene Proteins c-kit / metabolism Receptors, Transferrin / metabolism Receptors, Transforming Growth Factor beta / antagonists & inhibitors Receptors, Transforming Growth Factor beta / metabolism Signal Transduction Transforming Growth Factor beta1 / physiology* Up-Regulation* |
| IF | 2.126 |
| Times Cited | 21 |
| WOS Category | DEVELOPMENTAL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| Mice | RBRC00438 |