| RRC ID |
19145
|
| Author |
Sugiyama D, Kulkeaw K, Mizuochi C.
|
| Title |
TGF-beta-1 up-regulates extra-cellular matrix production in mouse hepatoblasts.
|
| Journal |
Mech Dev
|
| Abstract |
Fetal liver is the major embryonic hematopoietic organ and is extrinsically colonized by circulating hematopoietic stem cells (HSCs). Integrin beta-1 expression on HSCs is crucial for colonization, suggesting that interaction of Integrin beta-1 with extra-cellular matrix (ECM) factors promotes HSC adherence to fetal liver. However, little is known about how ECM production is regulated in fetal liver. Here we used flow cytometry to sort fetal liver compartments and detected ECM gene and protein expression predominantly in sorted hepatoblasts. mRNA and protein analysis suggested that TGF-beta-1 expressed by hepatoblasts, sinusoid endothelial cells and hematopoietic cells, binds to the TGF-beta receptor type-2 expressed on hepatoblasts to stimulate ECM production. Intra-cardiac injection of TGF-inhibitors into mouse embryos dramatically decreased fetal liver ECM gene expression. Taken together, our observations suggest that hepatoblasts predominantly produce ECM factors under control of TGF-beta-1 in fetal liver.
|
| Volume |
130(2-3)
|
| Pages |
195-206
|
| Published |
2013-2-1
|
| DOI |
10.1016/j.mod.2012.09.003
|
| PII |
S0925-4773(12)00088-3
|
| PMID |
23041440
|
| MeSH |
Animals
Antigens, CD / metabolism
Cells, Cultured
Embryo Culture Techniques
Extracellular Matrix / genetics
Extracellular Matrix / metabolism*
Extracellular Matrix Proteins / genetics
Extracellular Matrix Proteins / metabolism
Gene Expression
Gene Expression Regulation, Developmental
Hematopoietic Stem Cells / metabolism
Integrins / metabolism
Liver / cytology*
Liver / embryology
Liver / metabolism
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Mice, Transgenic
Proto-Oncogene Proteins c-kit / metabolism
Receptors, Transferrin / metabolism
Receptors, Transforming Growth Factor beta / antagonists & inhibitors
Receptors, Transforming Growth Factor beta / metabolism
Signal Transduction
Transforming Growth Factor beta1 / physiology*
Up-Regulation*
|
| IF |
2.126
|
| Times Cited |
21
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
| Resource |
| Mice |
RBRC00438 |
