論文 - 詳細
RRC ID | 19290 |
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著者 | Hassan F, Islam S, Tumurkhuu G, Dagvadorj J, Naiki Y, Komatsu T, Koide N, Yoshida T, Yokochi T. |
タイトル | Involvement of interleukin-1 receptor-associated kinase (IRAK)-M in toll-like receptor (TLR) 7-mediated tolerance in RAW 264.7 macrophage-like cells. |
ジャーナル | Cell Immunol |
Abstract |
The effect of toll-like receptor (TLR) 7 ligand pretreatment on the production of tumor necrosis factor (TNF)-alpha in response to TLR7 or TLR2 ligand was examined in order to establish a new TLR-mediated tolerance. RAW 264.7 macrophage-like cells were treated with imiquimod R837 as a TLR7 ligand for 18h, washed and incubated in fresh culture medium 6h. The second challenge with imiquimod R837 as a TLR7 ligand or Pam3CysSK4 as a TLR2 ligand resulted in reduced TNF-alpha production in TLR7 ligand-pretreated cells. There was impaired activation of NF-kappaB, p38 and stress-activated protein kinase (SAPK) in the tolerant cells. The expression of IRAK-M as a negative regulator of TLR signaling was markedly augmented in the tolerant cells while the interleukin-1 receptor-associated kinase (IRAK)-1 functioned normally. The involvement of IRAK-M in the TLR7-mediated tolerance is discussed. |
巻・号 | 256(1-2) |
ページ | 99-103 |
公開日 | 2009-1-1 |
DOI | 10.1016/j.cellimm.2009.01.013 |
PII | S0008-8749(09)00021-5 |
PMID | 19251253 |
MeSH | Aminoquinolines / pharmacology Animals Base Sequence Cell Line DNA Primers / genetics Imiquimod Immune Tolerance / physiology* Interleukin-1 Receptor-Associated Kinases / antagonists & inhibitors Interleukin-1 Receptor-Associated Kinases / genetics Interleukin-1 Receptor-Associated Kinases / immunology* Ligands Lipopeptides / pharmacology MAP Kinase Signaling System / drug effects MAP Kinase Signaling System / immunology Macrophages / drug effects Macrophages / immunology* Macrophages / metabolism Membrane Glycoproteins / immunology* Mice NF-kappa B / metabolism Nitric Oxide / biosynthesis RNA, Small Interfering / genetics Signal Transduction / drug effects Signal Transduction / immunology Toll-Like Receptor 2 / metabolism Toll-Like Receptor 7 / immunology* Tumor Necrosis Factor-alpha / biosynthesis p38 Mitogen-Activated Protein Kinases / metabolism |
IF | 4.078 |
引用数 | 17 |
WOS 分野 | IMMUNOLOGY CELL BIOLOGY |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |