| RRC ID |
19326
|
| Author |
Kasugai C, Morikawa A, Naiki Y, Koide N, Komatsu T, Yoshida T, Yokochi T.
|
| Title |
Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells.
|
| Journal |
Immunopharmacol Immunotoxicol
|
| Abstract |
The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate- resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.
|
| Volume |
31(1)
|
| Pages |
103-7
|
| Published |
2009-1-1
|
| DOI |
10.1080/08923970802438417
|
| PII |
908654651
|
| PMID |
19234958
|
| MeSH |
Acid Phosphatase / metabolism
Animals
Cell Differentiation / drug effects*
Cell Line
Concanavalin A / pharmacology*
Culture Media, Conditioned / pharmacology
Giant Cells / cytology
Giant Cells / metabolism
Isoenzymes / metabolism
Macrophages / cytology*
Macrophages / drug effects
Macrophages, Peritoneal / cytology
Macrophages, Peritoneal / drug effects
Mice
Mitogen-Activated Protein Kinases / antagonists & inhibitors
Osteoclasts / cytology*
Osteoclasts / metabolism
Phytohemagglutinins / pharmacology
Pokeweed Mitogens / pharmacology
Protein Kinase Inhibitors / pharmacology
Signal Transduction / drug effects
Tartrate-Resistant Acid Phosphatase
|
| IF |
2.352
|
| Times Cited |
4
|
|
WOS Category
|
PHARMACOLOGY & PHARMACY
TOXICOLOGY
IMMUNOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
