RRC ID |
19326
|
著者 |
Kasugai C, Morikawa A, Naiki Y, Koide N, Komatsu T, Yoshida T, Yokochi T.
|
タイトル |
Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells.
|
ジャーナル |
Immunopharmacol Immunotoxicol
|
Abstract |
The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate- resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.
|
巻・号 |
31(1)
|
ページ |
103-7
|
公開日 |
2009-1-1
|
DOI |
10.1080/08923970802438417
|
PII |
908654651
|
PMID |
19234958
|
MeSH |
Acid Phosphatase / metabolism
Animals
Cell Differentiation / drug effects*
Cell Line
Concanavalin A / pharmacology*
Culture Media, Conditioned / pharmacology
Giant Cells / cytology
Giant Cells / metabolism
Isoenzymes / metabolism
Macrophages / cytology*
Macrophages / drug effects
Macrophages, Peritoneal / cytology
Macrophages, Peritoneal / drug effects
Mice
Mitogen-Activated Protein Kinases / antagonists & inhibitors
Osteoclasts / cytology*
Osteoclasts / metabolism
Phytohemagglutinins / pharmacology
Pokeweed Mitogens / pharmacology
Protein Kinase Inhibitors / pharmacology
Signal Transduction / drug effects
Tartrate-Resistant Acid Phosphatase
|
IF |
2.352
|
引用数 |
4
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
TOXICOLOGY
IMMUNOLOGY
|
リソース情報 |
ヒト・動物細胞 |
RAW 264(RCB0535) |