RRC ID 19326
著者 Kasugai C, Morikawa A, Naiki Y, Koide N, Komatsu T, Yoshida T, Yokochi T.
タイトル Concanavalin A induces formation of osteoclast-like cells in RAW 264.7 mouse macrophage cells.
ジャーナル Immunopharmacol Immunotoxicol
Abstract The effect of lectins on the formation of osteoclasts in RAW 264.7 mouse macrophage cells was examined. Concanavalin A (Con A) induced the formation of multinucleated giant cells (MGC) whereas pokeweed mitogen and phytohemagglutinin did not do it. Con A-induced MGC were positive for tartrate- resistant acid phosphatase (TRAP) activity, a histochemical marker of osteoclasts. Murine splenic macrophages differentiated into TRAP-positive and multinucleated cells in response to Con A whereas peritoneal macrophages did not. The culture supernatant from Con A-stimulated RAW 264.7 cells did not cause the MGC formation. The relationship between Con A-induced GMC formation and osteoclastgenesis is discussed.
巻・号 31(1)
ページ 103-7
公開日 2009-1-1
DOI 10.1080/08923970802438417
PII 908654651
PMID 19234958
MeSH Acid Phosphatase / metabolism Animals Cell Differentiation / drug effects* Cell Line Concanavalin A / pharmacology* Culture Media, Conditioned / pharmacology Giant Cells / cytology Giant Cells / metabolism Isoenzymes / metabolism Macrophages / cytology* Macrophages / drug effects Macrophages, Peritoneal / cytology Macrophages, Peritoneal / drug effects Mice Mitogen-Activated Protein Kinases / antagonists & inhibitors Osteoclasts / cytology* Osteoclasts / metabolism Phytohemagglutinins / pharmacology Pokeweed Mitogens / pharmacology Protein Kinase Inhibitors / pharmacology Signal Transduction / drug effects Tartrate-Resistant Acid Phosphatase
IF 2.352
引用数 4
WOS 分野 PHARMACOLOGY & PHARMACY TOXICOLOGY IMMUNOLOGY
リソース情報
ヒト・動物細胞 RAW 264(RCB0535)