RRC ID 19332
著者 Tanaka T, Kawashima H, Ohnishi K, Matsumura K, Yoshimura R, Matsuyama M, Kuratsukuri K, Nakatani T.
タイトル Indirect antitumor effects of bisphosphonates on prostatic LNCaP cells co-cultured with bone cells.
ジャーナル Anticancer Res
Abstract Bisphosphonates are strong inhibitors of osteoclastic bone resorption in both benign and malignant bone diseases. The nitrogen-containing bisphosphonates (N-BPs) have strong cytotoxicity via inhibition of protein prenylation in the mevalonate pathway, and also demonstrate direct cytostatic and proapoptotic effects on prostate cancer cells. We confirmed the usefulness of a co-culture system comprised of prostatic LNCaP cells, ST2 cells (mouse-derived osteoblasts) and MLC-6 cells (mouse-derived osteoclasts) in vitro. N-BPs (pamidronate and zoledronic acid) inhibited both androgen receptor transactivation and tumor cell proliferation by suppressing the activities of both osteoclasts and osteoblasts with low-dose exposure. This indirect inhibition of prostate cancer cells via bone cells could be beneficial in treating prostate cancer patients with bone metastases.
巻・号 29(4)
ページ 1089-94
公開日 2009-4-1
PII 29/4/1089
PMID 19414350
MeSH Animals Antineoplastic Agents / pharmacology Bone Density Conservation Agents / pharmacology Cell Proliferation / drug effects Coculture Techniques Diphosphonates / pharmacology* Humans Imidazoles / pharmacology* Luciferases / metabolism Male Mice Osteoblasts / cytology Osteoclasts / cytology Pamidronate Prostatic Neoplasms / drug therapy* Prostatic Neoplasms / metabolism Prostatic Neoplasms / pathology RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Androgen / genetics Receptors, Androgen / metabolism Reverse Transcriptase Polymerase Chain Reaction Transcriptional Activation Tumor Cells, Cultured Zoledronic Acid
IF 1.994
引用数 5
WOS 分野 ONCOLOGY
リソース情報
ヒト・動物細胞 ST2(RCB0224) MLC-6(RCB1536)