RRC ID 19655
Author Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y.
Title Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1.
Journal Exp Cell Res
Abstract Mammals have 10 RASSF proteins, which are characterized by the Ras-association (RA) domain. Among them, RASSF1 to RASSF6 have the Salvador/RASSF/Hippo (SARAH) domain and form the subclass C-terminal RASSF proteins. Drosophila genome has a single C-terminal RASSF, dRASSF. All these RASSF proteins are related to the tumor suppressive Hippo pathway, and are considered to function as tumor suppressors. Caenorhabditis elegans T24F1.3 encodes a protein with the RA and the SARAH domains. The amino acid sequences are 40% and 55% similar to those of RASSF1 in the RA and the SARAH domains, respectively. We have characterized T24F1.3 gene product and named it RSF-1 as RASSF1 homolog. RSF-1 is widely expressed in epithelial cells. About 14% rsf-1 mutants exhibit defects in embryonal morphogenesis and the actin disorganization. The combinatorial synthetic lethal analysis demonstrates that the lethality is enhanced to more than 80% in rsf-1 mutants with the WASP-family verprolin homologous protein complex-related gene depletions and corroborates the implication of RSF-1 in the regulation of actin cytoskeleton. In rsf-1 mutants, the structures of muscle actin are preserved, but the swimming ability is impaired. Although we could not detect the direct physical interaction of LET-60 with RSF-1, rsf-1 mutants suppress the multivulva phenotype of the active let-60 mutants, suggesting that rsf-1 genetically interacts with the Ras signaling.
Volume 319(3)
Pages 1-11
Published 2013-2-1
DOI 10.1016/j.yexcr.2012.10.008
PII S0014-4827(12)00429-6
PMID 23103556
MeSH Actin Cytoskeleton / metabolism Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / isolation & purification Caenorhabditis elegans Proteins / metabolism Carrier Proteins / genetics* Cloning, Molecular Drosophila Proteins / genetics* Embryo, Nonmammalian HEK293 Cells Humans Models, Biological Molecular Sequence Data Protein Binding Sequence Homology Tumor Cells, Cultured ras Proteins / genetics ras Proteins / metabolism
IF 3.329
Times Cited 4
C.elegans tm5002