論文 - 詳細
| RRC ID | 2030 |
|---|---|
| 著者 | Morita N, Yasumori T, Nakayama K. |
| タイトル | Human MDR1 polymorphism: G2677T/A and C3435T have no effect on MDR1 transport activities. |
| ジャーナル | Biochem Pharmacol |
| Abstract |
The two most frequently observed single nucleotide polymorphisms (SNPs) of the human multidrug resistance 1 (MDR1) gene are 2677G/T/A (893Ala/Ser/Thr) and 3435C/T (no amino acid substitution). In this study, six forms of MDR1 cDNAs with the SNPs were expressed in LLC-PK1 cells and their transport activities were determined. Nearly identical amounts of the recombinant MDR1 proteins were expressed in the established cell lines using the Flp recombinase, which integrates a gene of interest at a specific genomic location. Four structurally diverse compounds: verapamil, digoxin, vinblastine and cyclosporin A, were examined for transcellular transport activities and intracellular accumulation. No significant differences were observed between cells expressing five polymorphic types of the MDR1 cDNAs (2677G/3435T, 2677A/3435C, 2677A/3435T, 2677T/3435C, 2677T/3435T) and cells expressing the wild-type (2677G/3435C). These results suggested that the two frequently observed MDR1 SNPs had no effect on the transport activities of MDR1 proteins expressed in LLC-PK1 cells in vitro, and other genetic or environmental factors might control the expression of MDR1 and the in vivo activity of MDR1. |
| 巻・号 | 65(11) |
| ページ | 1843-52 |
| 公開日 | 2003-6-1 |
| DOI | 10.1016/s0006-2952(03)00178-3 |
| PII | S0006295203001783 |
| PMID | 12781336 |
| MeSH | ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism* Animals Biological Transport Cells, Cultured Genome, Human Genotype* Humans Point Mutation Polymorphism, Single Nucleotide Recombinant Proteins / metabolism |
| IF | 4.96 |
| 引用数 | 101 |
| WOS 分野 | PHARMACOLOGY & PHARMACY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Human MDR1 cDNA (RDB01372) |