| RRC ID |
20386
|
| 著者 |
Makiuchi T, Nara T, Annoura T, Hashimoto T, Aoki T.
|
| タイトル |
Occurrence of multiple, independent gene fusion events for the fifth and sixth enzymes of pyrimidine biosynthesis in different eukaryotic groups.
|
| ジャーナル |
Gene
|
| Abstract |
The genes encoding orotate phosphoribosyltransferase (OPRT) and orotidine-5'-monophosphate decarboxylase (OMPDC), the fifth and sixth enzymes in the de novo pyrimidine biosynthetic pathway, are fused as OPRT-OMPDC in most eukaryotic groups. On the other hand, the inversely linked OMPDC-OPRT fusion is present in trypanosomatids, belonging to kinetoplastids together with bodonids in a supergroup, Euglenozoa. Here, we show the presence of OMPDC-OPRT in the bodonid, Bodo caudatus, while OPRT-OMPDC in Euglena gracilis, another euglenozoan species belonging to euglenoids. These results suggest that the OMPDC-OPRT fusion event occurred in a common ancestor of kinetoplastids. Genome sequence database searches further revealed the presence of OMPDC-OPRT in stramenopiles and cyanobacteria. Phylogenetic reconstruction of OPRT and OMPDC rejected statistically the monophyly of the OPRT domains of stramenopile and kinetoplastid OMPDC-OPRT, demonstrating that these gene fusions do not share a common evolutionary origin, despite the identical gene order. Thus, the OMPDC-OPRT fusion is likely to have emerged independently in these eukaryotic groups. Phylogenetic analyses also suggested that cyanobacterial OMPDC-OPRT arose via lateral transfer. We conclude that gene fusion events occur more frequently than previously thought and that lateral gene transfer has made a marked contribution to establishment of the rearranged structure of OPRT and OMPDC genes in eukaryotes.
|
| 巻・号 |
394(1-2)
|
| ページ |
78-86
|
| 公開日 |
2007-6-1
|
| DOI |
10.1016/j.gene.2007.02.009
|
| PII |
S0378-1119(07)00089-3
|
| PMID |
17383832
|
| MeSH |
Animals
Cloning, Molecular
Cyanobacteria / genetics
Cyanobacteria / metabolism
DNA, Algal / genetics
DNA, Complementary / genetics
DNA, Protozoan / genetics
Euglena gracilis / genetics*
Euglena gracilis / metabolism*
Evolution, Molecular
Gene Fusion*
Gene Transfer, Horizontal
Genes, Protozoan
Kinetoplastida / genetics*
Kinetoplastida / metabolism*
Models, Genetic
Molecular Sequence Data
Phylogeny
Pyrimidines / biosynthesis*
Species Specificity
|
| IF |
2.984
|
| 引用数 |
16
|
|
WOS 分野
|
GENETICS & HEREDITY
|
| リソース情報 |
| 藻類 |
NIES-48 |
