RRC ID 20961
Author Nguyen HN, Raisley B, Hadwiger JA.
Title MAP kinases have different functions in Dictyostelium G protein-mediated signaling.
Journal Cell. Signal.
Abstract Extracellular signal regulated kinases (ERKs) are a class of MAP kinases that function in many signaling pathways in eukaryotic cells and in some cases, a single stimulus can activate more than one ERK suggesting functional redundancy or divergence from a common pathway. Dictyostelium discoideum encodes only two MAP kinases, ERK1 and ERK2, that both function during the developmental life cycle. To determine if ERK1 and ERK2 have overlapping functions, chemotactic and developmental phenotypes of erk1(-) and erk2(-) mutants were assessed with respect to G protein-mediated signal transduction pathways. ERK1 was specifically required for Galpha5-mediated tip morphogenesis and inhibition of folate chemotaxis but not for cAMP-stimulated chemotaxis or cGMP accumulation. ERK2 was the primary MAPK phosphorylated in response to folate or cAMP stimulation. Cell growth was not altered in erk1(-), erk2(-) or erk1(-)erk2(-) mutants but each mutant displayed a different pattern of cell sorting in chimeric aggregates. The distribution of GFP-ERK1 or GFP-ERK2 fusion proteins in the cytoplasm and nucleus was not grossly altered in cells stimulated with cAMP or folate. These results suggest ERK1 and ERK2 have different roles in G protein-mediated signaling during growth and development.
Volume 22(5)
Pages 836-47
Published 2010-5
DOI 10.1016/j.cellsig.2010.01.008
PII S0898-6568(10)00014-8
PMID 20079430
PMC PMC2859692
MeSH Animals Cell Aggregation / drug effects Cell Shape / drug effects Cell Survival / drug effects Chemotaxis / drug effects Cyclic AMP / pharmacology Dictyostelium / cytology Dictyostelium / drug effects Dictyostelium / enzymology* Dictyostelium / growth & development Enzyme Activation / drug effects Folic Acid / pharmacology GTP-Binding Protein alpha Subunits / metabolism GTP-Binding Proteins / metabolism* MAP Kinase Signaling System* / drug effects Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Morphogenesis / drug effects Mutation / genetics Phosphorylation / drug effects Subcellular Fractions / drug effects Subcellular Fractions / enzymology Transformation, Genetic / drug effects
IF 3.487
Times Cited 5
Cellular slime molds G24686