論文 - 詳細
| RRC ID | 20969 |
|---|---|
| 著者 | Kuwayama H. |
| タイトル | Arachidonic acid enhances caffeine-induced cell death via caspase-independent cell death. |
| ジャーナル | Sci Rep |
| Abstract |
Caffeine is a globally consumed psychostimulant but can be fatal to cells at overdose exposures. Although caspase-dependent apoptosis plays a role in caffeine-induced cell death, the responsible intracellular signalling cascade remains incompletely understood. The cellular slime mould, Dictyostelium discoideum, does not possess caspase-dependent apoptotic machinery. Here, we observed that ablation of D. discoideumplaA, which encodes a phospholipase A2 (PLA₂) homolog, leads to a decreased rate of cell death under high caffeine concentrations and to enhanced cell death with the addition of arachidonic acid. Moreover, the inhibition of PLA₂ activity lead to a recovery of the survival rate in caspase-inhibited Hela cervical carcinoma cells under high caffeine concentrations, indicating that caffeine-induced cell death is enhanced via PLA₂-dependent signalling. Our results indicate that arachidonic acid may be a general second messenger that negatively regulates caffeine tolerance via a caspase-independent cell death cascade, which leads to multiple effects in eukaryotic cells. |
| 巻・号 | 2 |
| ページ | 577 |
| 公開日 | 2012-1-1 |
| DOI | 10.1038/srep00577 |
| PII | srep00577 |
| PMID | 22896810 |
| PMC | PMC3419364 |
| MeSH | Apoptosis / drug effects* Apoptosis / genetics Arachidonic Acid / pharmacology* Caffeine / pharmacology* Caspases / metabolism* Cell Survival / drug effects Cyclic AMP / metabolism Dictyostelium / drug effects Dictyostelium / genetics Dictyostelium / metabolism Drug Resistance Drug Synergism Enzyme Activation / drug effects HeLa Cells Humans Phospholipases A2 / metabolism Signal Transduction / drug effects |
| IF | 3.998 |
| 引用数 | 5 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | |
| 各媒体での言及数の合計 | 16 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | S00141 |