| RRC ID |
20977
|
| Author |
Umebayashi H, Mizokami A, Matsuda M, Harada K, Takeuchi H, Tanida I, Hirata M, Kanematsu T.
|
| Title |
Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Upon starvation, cells undergo autophagy, an intracellular bulk-degradation process, to provide the required nutrients. Here, we observed that phospholipase C-related catalytically inactive protein (PRIP) binds to microtubule-associated protein 1 light chain 3 (LC3), a mammalian autophagy-related initiator that regulates the autophagy pathway. Then, we examined the involvement of PRIP in the nutrient depletion-induced autophagy pathway. Enhanced colocalization of PRIP with LC3 was clearly seen in nutrient-starved mouse embryonic fibroblasts under a fluorescent microscope, and interaction of the proteins was revealed by immunoprecipitation experiments with an anti-LC3 antibody. Under starvation conditions, there were more green fluorescent protein fused-LC3 dots in mouse embryonic fibroblasts from PRIP-deficient mice than in fibroblasts from wild type cells. The formation of new dots in a single cell increased, as assessed by time-lapse microscopy. Furthermore, the increase in autophagosome formation in PRIP-deficient cells was notably inhibited by exogenously overexpressed PRIP. Taken together, PRIP is a novel LC3-binding protein that acts as a negative modulator of autophagosome formation.
|
| Volume |
432(2)
|
| Pages |
268-74
|
| Published |
2013-3-8
|
| DOI |
10.1016/j.bbrc.2013.01.119
|
| PII |
S0006-291X(13)00220-9
|
| PMID |
23399561
|
| MeSH |
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Amino Acid Sequence
Animals
Apoptosis Regulatory Proteins
Autophagy
Catalysis
Cells, Cultured
Cytoskeletal Proteins / metabolism
Fibroblasts / metabolism
Membrane Proteins / metabolism
Mice
Mice, Knockout
Microtubule-Associated Proteins / metabolism*
Molecular Sequence Data
Phagosomes
Type C Phospholipases / metabolism
|
| IF |
2.985
|
| Times Cited |
6
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Mice |
RBRC00806 |
