Reference - Detail
|Author||Oku M, Hoseki J, Ichiki Y, Sakai Y.|
|Title||A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae.|
The physiological roles of the thioredoxin isozymes in the yeast Saccharomyces cerevisiae were investigated using a novel FRET-based redox probe, Redoxfluor. After establishing responsiveness of the probe toward thioredoxin, we followed the fluorescence signal of Redoxfluor expressed in the yeast and found that one of the thioredoxin isozymes, Trx2, was required for maintaining the redox status when stationary culture of the organism was exposed to starvation and mild-heat stresses. The failure to maintain redox balance under the tested condition preceded decreased viability of the trx2 mutants, indicating the functional importance of the cytoplasmic thioredoxin in adaptation to environmental changes.
|MeSH||Biosensing Techniques Fluorescence Resonance Energy Transfer Fluorescent Dyes / chemistry Fluorescent Dyes / metabolism* Gene Deletion Gene Expression Isoenzymes / genetics Isoenzymes / metabolism Microbial Viability Microscopy, Fluorescence Oxidation-Reduction Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism* Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Starvation Stress, Physiological Thioredoxins / genetics Thioredoxins / metabolism*|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|