論文 - 詳細
| RRC ID | 20982 |
|---|---|
| 著者 | Ikeshima-Kataoka H, Abe Y, Abe T, Yasui M. |
| タイトル | Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin. |
| ジャーナル | Mol Cell Neurosci |
| Abstract |
During injury to the central nervous system (CNS), astrocytes and microglia proliferate and migrate around the lesion sites. Recently, it has been reported that one of the water channels, aquaporin-4 (AQP4) is seemed to have a role in astroglial migration and glial scar formation caused by brain injury, although its molecular mechanism is largely unknown. In the present study, we examined the expression profiles in wild-type (WT) and AQP4-deficient (AQP4/KO) mice after a stab wound to the cerebral cortex. Three days after the stab wound, AQP4 expression was observed in activated microglia around the lesion site as well as in astrocytes. A microarray analysis revealed that 444 genes around the lesion site were upregulated 3 days after the wounding in WT mice. Surprisingly, most of these up-regulations were significantly attenuated in AQP4/KO mice. Real-time RT-PCR and immunofluorescence showed that osteopontin (OPN) expression around the lesion site was much lower in AQP4/KO mice than in WT mice. Moreover, the up-regulation of pro-inflammatory cytokines was significantly attenuated in AQP4/KO mice. Taken together, these results suggest that AQP4 plays an important role in immunological function in concert with OPN under pathological conditions in the CNS. |
| 巻・号 | 56 |
| ページ | 65-75 |
| 公開日 | 2013-9-1 |
| DOI | 10.1016/j.mcn.2013.02.002 |
| PII | S1044-7431(13)00020-1 |
| PMID | 23428384 |
| MeSH | Animals Aquaporin 4 / genetics Aquaporin 4 / metabolism* Astrocytes / metabolism Cerebral Cortex / injuries* Cerebral Cortex / metabolism Cerebral Cortex / pathology Gene Expression Profiling Inflammation / genetics Inflammation / metabolism Mice Mice, Inbred C57BL Mice, Knockout Microglia / metabolism Osteopontin / genetics Osteopontin / metabolism* Up-Regulation Wounds, Stab / immunology Wounds, Stab / metabolism* Wounds, Stab / pathology |
| IF | 3.182 |
| 引用数 | 27 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC01835 |