RRC ID 21261
著者 Gourguechon S, Holt LJ, Cande WZ.
タイトル The Giardia cell cycle progresses independently of the anaphase-promoting complex.
ジャーナル J Cell Sci
Abstract Most cell cycle regulation research has been conducted in model organisms representing a very small part of the eukaryotic domain. The highly divergent human pathogen Giardia intestinalis is ideal for studying the conservation of eukaryotic pathways. Although Giardia has many cell cycle regulatory components, its genome lacks all anaphase-promoting complex (APC) components. In the present study, we show that a single mitotic cyclin in Giardia is essential for progression into mitosis. Strikingly, Giardia cyclin B lacks the conserved N-terminal motif required for timely degradation mediated by the APC and ubiquitin conjugation. Expression of Giardia cyclin B in fission yeast is toxic, leading to a prophase arrest, and this toxicity is suppressed by the addition of a fission yeast degradation motif. Cyclin B is degraded during mitosis in Giardia cells, but this degradation appears to be independent of the ubiquitination pathway. Other putative APC substrates, aurora and polo-like kinases, also show no evidence of ubiquitination. This is the first example of mitosis not regulated by the APC and might reflect an evolutionary ancient form of cell cycle regulation.
巻・号 126(Pt 10)
ページ 2246-55
公開日 2013-5-15
DOI 10.1242/jcs.121632
PII jcs.121632
PMID 23525017
PMC PMC3672939
MeSH Anaphase-Promoting Complex-Cyclosome / genetics Biological Evolution Cell Cycle / genetics Conserved Sequence / genetics Cyclin B / genetics Cyclin B / metabolism* Giardia lamblia / physiology* Morpholinos / genetics Phosphorylation Protein Structure, Tertiary / genetics Proteolysis Protozoan Proteins / genetics Protozoan Proteins / metabolism* Schizosaccharomyces Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Species Specificity Ubiquitination
IF 4.573
引用数 29
WOS 分野 CELL BIOLOGY
リソース情報
酵母 Cdc13-100