RRC ID 21412
著者 Wada R, Maruyama J, Yamaguchi H, Yamamoto N, Wagu Y, Paoletti M, Archer DB, Dyer PS, Kitamoto K.
タイトル Presence and functionality of mating type genes in the supposedly asexual filamentous fungus Aspergillus oryzae.
ジャーナル Appl Environ Microbiol
Abstract The potential for sexual reproduction in Aspergillus oryzae was assessed by investigating the presence and functionality of MAT genes. Previous genome studies had identified a MAT1-1 gene in the reference strain RIB40. We now report the existence of a complementary MAT1-2 gene and the sequencing of an idiomorphic region from A. oryzae strain AO6. This allowed the development of a PCR diagnostic assay, which detected isolates of the MAT1-1 and MAT1-2 genotypes among 180 strains assayed, including industrial tane-koji isolates. Strains used for sake and miso production showed a near-1:1 ratio of the MAT1-1 and MAT1-2 mating types, whereas strains used for soy sauce production showed a significant bias toward the MAT1-2 mating type. MAT1-1 and MAT1-2 isogenic strains were then created by genetic manipulation of the resident idiomorph, and gene expression was compared by DNA microarray and quantitative real-time PCR (qRT-PCR) methodologies under conditions in which MAT genes were expressed. Thirty-three genes were found to be upregulated more than 10-fold in either the MAT1-1 host strain or the MAT1-2 gene replacement strain relative to each other, showing that both the MAT1-1 and MAT1-2 genes functionally regulate gene expression in A. oryzae in a mating type-dependent manner, the first such report for a supposedly asexual fungus. MAT1-1 expression specifically upregulated an α-pheromone precursor gene, but the functions of most of the genes affected were unknown. The results are consistent with a heterothallic breeding system in A. oryzae, and prospects for the discovery of a sexual cycle are discussed.
巻・号 78(8)
ページ 2819-29
公開日 2012-4-1
DOI 10.1128/AEM.07034-11
PII AEM.07034-11
PMID 22327593
PMC PMC3318824
MeSH Amino Acid Sequence Aspergillus oryzae / genetics Aspergillus oryzae / growth & development Aspergillus oryzae / isolation & purification Aspergillus oryzae / physiology* DNA Primers / genetics DNA, Fungal / chemistry DNA, Fungal / genetics Food Microbiology Fungi / genetics Fungi / growth & development Fungi / physiology Gene Deletion Gene Expression Profiling Genes, Mating Type, Fungal* Genotype Microarray Analysis Molecular Sequence Data Oligonucleotide Array Sequence Analysis Real-Time Polymerase Chain Reaction Sequence Analysis, DNA Sequence Homology, Amino Acid
IF 4.016
引用数 37
WOS 分野 BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
リソース情報
一般微生物 JCM 2058 JCM 2247 JCM 12736 JCM 22393 JCM 22428