RRC ID 21510
著者 Ansai S, Ochiai H, Kanie Y, Kamei Y, Gou Y, Kitano T, Yamamoto T, Kinoshita M.
タイトル Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases.
ジャーナル Dev Growth Differ
Abstract Zinc-finger nucleases (ZFNs) are artificial enzymes that create site-specific double-strand breaks and thereby induce targeted genome editing. Here, we demonstrated successful gene disruption in somatic and germ cells of medaka (Oryzias latipes) using ZFN to target exogenous EGFP genes. Embryos that were injected with an RNA sequence pair coding for ZFNs showed mosaic loss of green fluorescent protein fluorescence in skeletal muscle. A number of mutations that included both deletions and insertions were identified within the ZFN target site in each embryo, whereas no mutations were found at the non-targeted sites. In addition, ZFN-induced mutations were introduced in germ cells and efficiently transmitted to the next generation. The mutation frequency varied (6-100%) in the germ cells from each founder, and a founder carried more than two types of mutation in germ cells. Our results have introduced the possibility of targeted gene disruption and reverse genetics in medaka.
巻・号 54(5)
ページ 546-56
公開日 2012-6-1
DOI 10.1111/j.1440-169X.2012.01357.x
PMID 22642582
MeSH Animals DNA Breaks, Double-Stranded Endonucleases / metabolism* Gene Targeting / methods* Germ-Line Mutation Green Fluorescent Proteins / genetics* Mutagenesis, Insertional Mutagenesis, Site-Directed / methods* Mutation Rate Oryzias / genetics* Sequence Deletion Zinc Fingers
IF 1.723
引用数 28
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
メダカ Tg(olvas-GFP) (TG870) d-rR/TOKYO (MT837) Database