RRC ID 21785
Author Nishibe R, Watanabe W, Ueda T, Yamasaki N, Koller R, Wolff L, Honda Z, Ohtsubo M, Honda H.
Title CIZ1, a p21Cip1/Waf1-interacting protein, functions as a tumor suppressor in vivo.
Journal FEBS Lett.
Abstract CIZ1 is a nuclear protein involved in DNA replication and is also implicated in human diseases including cancers. To gain an insight into its function in vivo, we generated mice lacking Ciz1. Ciz1-deficient (Ciz1(-/-)) mice grew without any obvious abnormalities, and Ciz1(-/-) mouse embryonic fibroblasts (MEFs) did not show any defects in cell cycle status, cell growth, and DNA damage response. However, Ciz1(-/-) MEFs were sensitive to hydroxyurea-mediated replication stress and susceptible to oncogene-induced cellular transformation. In addition, Ciz1(-/-) mice developed various types of leukemias by retroviral insertional mutagenesis. These results indicate that CIZ1 functions as a tumor suppressor in vivo.
Volume 587(10)
Pages 1529-35
Published 2013-5-21
DOI 10.1016/j.febslet.2013.03.034
PII S0014-5793(13)00263-9
PMID 23583447
MeSH Animals Ataxia Telangiectasia Mutated Proteins Cell Cycle / genetics Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Proliferation Cell Transformation, Neoplastic / genetics Cell Transformation, Neoplastic / metabolism Cells, Cultured Cyclin-Dependent Kinase Inhibitor p21 / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Embryo, Mammalian / cytology Fibroblasts / metabolism Fibroblasts / physiology Mice Mice, Knockout Models, Biological Nuclear Proteins / genetics Nuclear Proteins / metabolism Nuclear Proteins / physiology* Protein Binding Protein-Serine-Threonine Kinases / genetics Protein-Serine-Threonine Kinases / metabolism Tumor Suppressor Proteins* / genetics Tumor Suppressor Proteins* / metabolism Tumor Suppressor Proteins* / physiology
IF 2.999
Times Cited 7
Mice B6.Cg-Tg(CAG-cre)CZ-MO2Osb (RBRC01828)