RRC ID 21837
著者 Schmidt I, Thomas S, Kain P, Risse B, Naffin E, Klämbt C.
タイトル Kinesin heavy chain function in Drosophila glial cells controls neuronal activity.
ジャーナル J Neurosci
Abstract Kinesin heavy chain (Khc) is crucially required for axonal transport and khc mutants show axonal swellings and paralysis. Here, we demonstrate that in Drosophila khc is equally important in glial cells. Glial-specific downregulation of khc by RNA interference suppresses neuronal excitability and results in spastic flies. The specificity of the phenotype was verified by interspecies rescue experiments and further mutant analyses. Khc is mostly required in the subperineurial glia forming the blood-brain barrier. Following glial-specific knockdown, peripheral nerves are swollen with maldistributed mitochondria. To better understand khc function, we determined Khc-dependent Rab proteins in glia and present evidence that Neurexin IV, a well known blood-brain barrier constituent, is one of the relevant cargo proteins. Our work shows that the role of Khc for neuronal excitability must be considered in the light of its necessity for directed transport in glia.
巻・号 32(22)
ページ 7466-76
公開日 2012-5-30
DOI 10.1523/JNEUROSCI.0349-12.2012
PII 32/22/7466
PMID 22649226
PMC PMC6703594
MeSH Animals Animals, Genetically Modified Axonal Transport / genetics Down-Regulation / genetics Down-Regulation / physiology* Drosophila Drosophila Proteins / genetics Electric Stimulation Kinesins / genetics Kinesins / metabolism* Larva Locomotion / genetics Locomotion / physiology Luminescent Proteins / genetics Luminescent Proteins / metabolism Male Membrane Potentials / genetics Neuroglia / metabolism* Neurons / physiology* Patch-Clamp Techniques Peripheral Nerves / cytology RNA Interference / physiology rab GTP-Binding Proteins / metabolism
IF 5.674
引用数 32
WOS 分野 NEUROSCIENCES
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