論文 - 詳細
| RRC ID | 21871 |
|---|---|
| 著者 | De Graeve FM, Van de Bor V, Ghiglione C, Cerezo D, Jouandin P, Ueda R, Shashidhara LS, Noselli S. |
| タイトル | Drosophila apc regulates delamination of invasive epithelial clusters. |
| ジャーナル | Dev Biol |
| Abstract |
Border Cells in the Drosophila ovaries are a useful genetic model for understanding the molecular events underlying epithelial cell motility. During stage 9 of egg chamber development they detach from neighboring stretched cells and migrate between the nurse cells to reach the oocyte. RNAi screening allowed us to identify the dapc1 gene as being critical in this process. Clonal and live analysis showed a requirement of dapc1 in both outer border cells and contacting stretched cells for delamination. This mutant phenotype was rescued by dapc1 or dapc2 expression. Loss of dapc1 function was associated with an abnormal lasting accumulation of β-catenin/Armadillo and E-cadherin at the boundary between migrating border and stretched cells. Moreover, β-catenin/armadillo or E-cadherin downregulation rescued the dapc1 loss of function phenotype. Altogether these results indicate that Drosophila Apc1 is required for dynamic remodeling of β-catenin/Armadillo and E-cadherin adhesive complexes between outer border cells and stretched cells regulating proper delamination and invasion of migrating epithelial clusters. |
| 巻・号 | 368(1) |
| ページ | 76-85 |
| 公開日 | 2012-8-1 |
| DOI | 10.1016/j.ydbio.2012.05.017 |
| PII | S0012-1606(12)00264-3 |
| PMID | 22627290 |
| MeSH | Animals Animals, Genetically Modified Armadillo Domain Proteins / genetics Armadillo Domain Proteins / metabolism Cadherins / genetics Cadherins / metabolism Cell Adhesion Cell Movement Cytoskeletal Proteins Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Epithelial Cells / cytology Epithelial Cells / metabolism* Female Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Immunohistochemistry Microscopy, Confocal Mutation Oocytes / cytology Oocytes / metabolism Ovary / cytology Ovary / metabolism* RNA Interference Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism* beta Catenin / genetics beta Catenin / metabolism |
| IF | 2.896 |
| 引用数 | 8 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ショウジョウバエ | 1451R-1 1451R-3 |