論文 - 詳細
| RRC ID | 21895 |
|---|---|
| 著者 | Kain P, Badsha F, Hussain SM, Nair A, Hasan G, Rodrigues V. |
| タイトル | Mutants in phospholipid signaling attenuate the behavioral response of adult Drosophila to trehalose. |
| ジャーナル | Chem Senses |
| Abstract |
In Drosophila melanogaster, gustatory receptor genes (Grs) encode putative G-protein-coupled receptors (GPCRs) that are expressed in gustatory receptor neurons (GRNs). One of the Gr genes, Gr5a, encodes a receptor for trehalose that is expressed in a subset of GRNs. Although a role for the G protein, Gsα, has been shown in Gr5a-expressing taste neurons, there is the residual responses to trehalose in Gsα mutants which could suggest additional transduction mechanisms. Expression and genetic analysis of the heterotrimeric G-protein subunit, Gq, shown here suggest involvement of this Gα subunit in trehalose perception in Drosophila. A green fluorescent protein reporter of Gq expression is detected in gustatory neurons in the labellum, tarsal segments, and wing margins. Animals heterozygous for dgq mutations and RNA interference-mediated knockdown of dgq showed reduced responses to trehalose in the proboscis extension reflex assay and feeding behavior assay. These defects were rescued by targeted expression of the wild-type dgqα transgene in the GRNs. These data together with observations from other mutants in phospholipid signaling provide insights into the mechanisms of taste transduction in Drosophila. |
| 巻・号 | 35(8) |
| ページ | 663-73 |
| 公開日 | 2010-10-1 |
| DOI | 10.1093/chemse/bjq055 |
| PII | bjq055 |
| PMID | 20543015 |
| MeSH | Animals Behavior, Animal* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / drug effects Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Female GTP-Binding Protein alpha Subunits, Gq-G11 / genetics* GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism Heterozygote Male Mutation RNA Interference Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Signal Transduction TRPC Cation Channels / genetics TRPC Cation Channels / metabolism Taste / physiology Trehalose / pharmacology* |
| IF | 2.261 |
| 引用数 | 15 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY PHYSIOLOGY BEHAVIORAL SCIENCES NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ショウジョウバエ | 1063R-1 1063R-2 |