RRC ID 21895
Author Kain P, Badsha F, Hussain SM, Nair A, Hasan G, Rodrigues V.
Title Mutants in phospholipid signaling attenuate the behavioral response of adult Drosophila to trehalose.
Journal Chem. Senses
Abstract In Drosophila melanogaster, gustatory receptor genes (Grs) encode putative G-protein-coupled receptors (GPCRs) that are expressed in gustatory receptor neurons (GRNs). One of the Gr genes, Gr5a, encodes a receptor for trehalose that is expressed in a subset of GRNs. Although a role for the G protein, Gsα, has been shown in Gr5a-expressing taste neurons, there is the residual responses to trehalose in Gsα mutants which could suggest additional transduction mechanisms. Expression and genetic analysis of the heterotrimeric G-protein subunit, Gq, shown here suggest involvement of this Gα subunit in trehalose perception in Drosophila. A green fluorescent protein reporter of Gq expression is detected in gustatory neurons in the labellum, tarsal segments, and wing margins. Animals heterozygous for dgq mutations and RNA interference-mediated knockdown of dgq showed reduced responses to trehalose in the proboscis extension reflex assay and feeding behavior assay. These defects were rescued by targeted expression of the wild-type dgqα transgene in the GRNs. These data together with observations from other mutants in phospholipid signaling provide insights into the mechanisms of taste transduction in Drosophila.
Volume 35(8)
Pages 663-73
Published 2010-10
DOI 10.1093/chemse/bjq055
PII bjq055
PMID 20543015
MeSH Animals Behavior, Animal* Drosophila Proteins / genetics* Drosophila Proteins / metabolism Drosophila melanogaster / drug effects Drosophila melanogaster / genetics Drosophila melanogaster / physiology* Female GTP-Binding Protein alpha Subunits, Gq-G11 / genetics* GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism Heterozygote Male Mutation RNA Interference Receptors, Cell Surface / genetics Receptors, Cell Surface / metabolism Signal Transduction TRPC Cation Channels / genetics TRPC Cation Channels / metabolism Taste / physiology Trehalose / pharmacology*
IF 2.52
Times Cited 12
WOS Category FOOD SCIENCE & TECHNOLOGY PHYSIOLOGY BEHAVIORAL SCIENCES NEUROSCIENCES
Resource
Drosophila 1063R-1 1063R-2