論文 - 詳細
| RRC ID | 21938 |
|---|---|
| 著者 | Warner SJ, Longmore GD. |
| タイトル | Distinct functions for Rho1 in maintaining adherens junctions and apical tension in remodeling epithelia. |
| ジャーナル | J Cell Biol |
| Abstract |
Maintenance and remodeling of adherens junctions (AJs) and cell shape in epithelia are necessary for the development of functional epithelia and are commonly altered during cancer progression/metastasis. Although formation of nascent AJs has received much attention, whether shared mechanisms are responsible for the maintenance and remodeling of AJs in dynamic epithelia, particularly in vivo, is not clear. Using clonal analysis in the postmitotic Drosophila melanogaster pupal eye epithelium, we demonstrate that Rho1 is required to maintain AJ integrity independent of its role in sustaining apical cell tension. Rho1 depletion in a remodeling postmitotic epithelium disrupts AJs but only when depleted in adjacent cells. Surprisingly, neither of the Rho effectors, Rok or Dia, is necessary downstream of Rho1 to maintain AJs; instead, Rho1 maintains AJs by inhibiting Drosophila epithelial cadherin endocytosis in a Cdc42/Par6-dependent manner. In contrast, depletion of Rho1 in single cells decreases apical tension, and Rok and myosin are necessary, while Dia function also contributes, downstream of Rho1 to sustain apical cell tension. |
| 巻・号 | 185(6) |
| ページ | 1111-25 |
| 公開日 | 2009-6-15 |
| DOI | 10.1083/jcb.200901029 |
| PII | jcb.200901029 |
| PMID | 19506041 |
| PMC | PMC2711606 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism Adherens Junctions / metabolism* Adherens Junctions / ultrastructure Animals Armadillo Domain Proteins / genetics Armadillo Domain Proteins / metabolism Cadherins / genetics Cadherins / metabolism Carrier Proteins / genetics Carrier Proteins / metabolism Cell Membrane / metabolism Cell Polarity Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / cytology Drosophila melanogaster / growth & development Drosophila melanogaster / physiology Epithelial Cells / cytology Epithelial Cells / metabolism* Epithelium / metabolism* Formins Isoenzymes / genetics Isoenzymes / metabolism Myosins / genetics Myosins / metabolism Photoreceptor Cells, Invertebrate / cytology Photoreceptor Cells, Invertebrate / metabolism RNA Interference Stress, Mechanical Transcription Factors / genetics Transcription Factors / metabolism cdc42 GTP-Binding Protein / genetics cdc42 GTP-Binding Protein / metabolism rho GTP-Binding Proteins / genetics rho GTP-Binding Proteins / metabolism* rho-Associated Kinases / genetics rho-Associated Kinases / metabolism |
| IF | 8.811 |
| リソース情報 | |
| ショウジョウバエ | 2055R-1 2055R-2 |