RRC ID 21946
著者 Gunawan F, Arandjelovic M, Godt D.
タイトル The Maf factor Traffic jam both enables and inhibits collective cell migration in Drosophila oogenesis.
ジャーナル Development
Abstract Border cell cluster (BCC) migration in the Drosophila ovary is an excellent system to study the gene regulatory network that enables collective cell migration. Here, we identify the large Maf transcription factor Traffic jam (Tj) as an important regulator of BCC migration. Tj has a multifaceted impact on the known core cascade that enables BCC motility, consisting of the Jak/Stat signaling pathway, the C/EBP factor Slow border cells (Slbo), and the downstream effector DE-cadherin (DEcad). The initiation of BCC migration coincides with a Slbo-dependent decrease in Tj expression. This reduction of Tj is required for normal BCC motility, as high Tj expression strongly impedes migration. At high concentration, Tj has a tripartite negative effect on the core pathway: a decrease in Slbo, an increase in the Jak/Stat inhibitor Socs36E, and a Slbo-independent reduction of DEcad. However, maintenance of a low expression level of Tj in the BCC during migration is equally important, as loss of tj function also results in a significant delay in migration concomitant with a reduction of Slbo and consequently of DEcad. Taken together, we conclude that the regulatory feedback loop between Tj and Slbo is necessary for achieving the correct activity levels of migration-regulating factors to ensure proper BCC motility.
巻・号 140(13)
ページ 2808-17
公開日 2013-7-1
DOI 10.1242/dev.089896
PII dev.089896
PMID 23720044
MeSH Animals CCAAT-Enhancer-Binding Proteins / genetics CCAAT-Enhancer-Binding Proteins / metabolism Cadherins / genetics Cadherins / metabolism Cell Movement / genetics Cell Movement / physiology Drosophila / embryology* Drosophila / metabolism* Drosophila Proteins / genetics Drosophila Proteins / metabolism* Female Maf Transcription Factors, Large / genetics Maf Transcription Factors, Large / metabolism* Oogenesis / genetics Oogenesis / physiology* Ovary / cytology Ovary / embryology* Ovary / metabolism Proto-Oncogene Proteins / genetics Proto-Oncogene Proteins / metabolism*
IF 5.611
引用数 10
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ショウジョウバエ 10034R-2