RRC ID 21970
Author Park JS, Lee SH, Na HJ, Pyo JH, Kim YS, Yoo MA.
Title Age- and oxidative stress-induced DNA damage in Drosophila intestinal stem cells as marked by Gamma-H2AX.
Journal Exp. Gerontol.
Abstract A decline in stem cell function is considered as a major cause of tissue atrophy, organ-system failure, cancer development and aging process. For a better understanding of the mechanism underlying age-related decline of stem cell function, characterization of aged stem cells is required. DNA damage induces epigenetic modifications that are associated with cell dysfunction. In mammals, γH2AX has been shown as DNA damage marker and an adaptor for recruiting chromatin modifying factors. In current study, utilizing a well-accepted Drosophila midgut model for stem-cell biology, we demonstrated aging- and oxidative stress-related accumulation of γH2AvD foci, analogous to mammal γH2AX, in Drosophila intestinal stem cells (ISCs), and obtained evidence that the changes in γH2AvD is closely associated with γ-ray-induced DNA damage in ISCs and age-related accumulation of 8-oxo-2'-deoxyguanosine. The significance of our study is to document the first direct evidence for the accumulation of age-related DNA-damage in ISCs, and to show γH2AvD as a useful biomarker in exploring the molecular mechanisms underlying stem cell aging in the Drosophila midgut.
Volume 47(5)
Pages 401-5
Published 2012-5
DOI 10.1016/j.exger.2012.02.007
PII S0531-5565(12)00038-1
PMID 22387531
MeSH Aging / genetics Aging / metabolism Aging / pathology Animals Biomarkers / metabolism DNA Damage / physiology* Drosophila / cytology Drosophila / genetics* Drosophila / metabolism Drosophila Proteins / metabolism Female Gamma Rays Histones / metabolism Intestines / cytology* Intestines / metabolism Intestines / radiation effects Male Oxidative Stress / genetics* Oxidative Stress / physiology Stem Cells / cytology* Stem Cells / radiation effects
IF 3.224
Times Cited 12
WOS Category GERIATRICS & GERONTOLOGY
Resource
Drosophila esg-GAL4 UAS-GFP/CyO