Reference - Detail
| RRC ID | 2200 |
|---|---|
| Author | Kasama T, Shigehisa A, Hirata A, Saito TT, Tougan T, Okuzaki D, Nojima H. |
| Title | Spo5/Mug12, a putative meiosis-specific RNA-binding protein, is essential for meiotic progression and forms Mei2 dot-like nuclear foci. |
| Journal | Eukaryot Cell |
| Abstract |
We report here a functional analysis of spo5(+)(mug12(+)) of Schizosaccharomyces pombe, which encodes a putative RNA-binding protein. The disruption of spo5(+) caused abnormal sporulation, generating inviable spores due to failed forespore membrane formation and the absence of a spore wall, as determined by electron microscopy. Spo5 regulates the progression of meiosis I because spo5 mutant cells display normal premeiotic DNA synthesis and the timely initiation of meiosis I but they show a delay in the peaking of cells with two nuclei, abnormal tyrosine 15 dephosphorylation of Cdc2, incomplete degradation of Cdc13, retarded formation and repair of double strand breaks, and a reduced frequency of intragenic recombination. Immunostaining showed that Spo5-green fluorescent protein (GFP) appeared in the cytoplasm at the horsetail phase, peaked around the metaphase I to anaphase I transition, and suddenly disappeared after anaphase II. Images of Spo5-GFP in living cells revealed that Spo5 forms a dot in the nucleus at prophase I that colocalized with the Mei2 dot. Unlike the Mei2 dot, however, the Spo5 dot was observed even in sme2Delta cells. Taken together, we conclude that Spo5 is a novel regulator of meiosis I and that it may function in the vicinity of the Mei2 dot. |
| Volume | 5(8) |
| Pages | 1301-13 |
| Published | 2006-8-1 |
| DOI | 10.1128/EC.00099-06 |
| PII | 5/8/1301 |
| PMID | 16896214 |
| PMC | PMC1539142 |
| MeSH | Amino Acid Sequence Meiosis* Molecular Sequence Data Mutation RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism* Schizosaccharomyces / genetics Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism* Sequence Alignment Spores, Fungal / metabolism Spores, Fungal / ultrastructure |
| IF | 2.992 |
| Times Cited | 17 |
| WOS Category | MYCOLOGY MICROBIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Yeast | CD16-1 FY9907(TP4-5A) FY12692(JZ670) FY16513(AS29) FY16511(AS17) FY16512(AS18) FY12710(YN68) FY16514(AS44) FY16515(AS24) FY16516(AS47) FY16517(AS7) FY16518(TK155) |