RRC ID 2201
Author Saito TT, Okuzaki D, Nojima H.
Title Mcp5, a meiotic cell cortex protein, is required for nuclear movement mediated by dynein and microtubules in fission yeast.
Journal J Cell Biol
Abstract During meiotic prophase I of the fission yeast Schizosaccharomyces pombe, oscillatory nuclear movement occurs. This promotes homologous chromosome pairing and recombination and involves cortical dynein, which plays a pivotal role by generating a pulling force with the help of an unknown dynein anchor. We show that Mcp5, the homologue of the budding yeast dynein anchor Num1, may be this putative dynein anchor. mcp5+ is predominantly expressed during meiotic prophase, and GFP-Mcp5 localizes at the cell cortex. Moreover, the mcp5Delta strain lacks the oscillatory nuclear movement. Accordingly, homologous pairing and recombination rates of the mcp5Delta strain are significantly reduced. Furthermore, the cortical localization of dynein heavy chain 1 appears to be reduced in mcp5Delta cells. Finally, the full function of Mcp5 requires its coiled-coil and pleckstrin homology (PH) domains. Our results suggest that Mcp5 localizes at the cell cortex through its PH domain and functions as a dynein anchor, thereby facilitating nuclear oscillation.
Volume 173(1)
Pages 27-33
Published 2006-4-10
DOI 10.1083/jcb.200512129
PII jcb.200512129
PMID 16585273
PMC PMC2063782
MeSH Biological Clocks / physiology Calcium-Binding Proteins / chemistry Calcium-Binding Proteins / genetics Calcium-Binding Proteins / metabolism Carrier Proteins Cell Nucleus / genetics Cell Nucleus / metabolism* Cytokinesis / physiology Cytoplasm / genetics Cytoplasm / metabolism Cytoskeletal Proteins Dyneins / genetics Dyneins / metabolism* Meiosis / physiology* Microtubules / genetics Microtubules / metabolism* Protein Structure, Tertiary / physiology Saccharomyces cerevisiae Proteins / chemistry Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism* Schizosaccharomyces pombe Proteins / chemistry Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism*
IF 8.811
Times Cited 31
Yeast CD16-1 FY16884(ST316-1) FY16886(SST318-1) FY7051(JZ670) FY16707(NP43-20) FY16848(ST259) FY12153(TP4-1D) FY15563(YY105) FY16826(ST218-1) FY16844(ST254) FY16896(ST349)